Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

Research output: Contribution to journalReviewResearchpeer-review

DOI

  1. The impact of concurrent HIV and type II diabetes on immune maturation, immune regulation and immune activation

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Epigenetic therapy in hematological cancers

    Research output: Contribution to journalReviewResearchpeer-review

  3. An update on head and neck cancer: new entities and their histopathology, molecular background, treatment, and outcome

    Research output: Contribution to journalReviewResearchpeer-review

  4. Subtyping of intraductal papillary mucinous neoplasms - pitfalls of MUC1 immunohistochemistry

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Mycobacterium brisbanense lung infection facilitated by steroid induced adrenal insufficiency

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. In-vivo gentamicin susceptibility test for prevention of bacterial biofilms in bone tissue and on implants

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. UV light assisted antibiotics for eradication of in vitro biofilms

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Biofilm formation - what we can learn from recent developments

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Bacterial biofilm formation inside colonic crypts may accelerate colorectal carcinogenesis

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Implants induce a new niche for microbiomes

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections. Thereby providing the first guideline for the morphology and identification of fluorescently stained PMNs, bacteria and biofilm.

Original languageEnglish
JournalAPMIS - Journal of Pathology, Microbiology and Immunology
Volume126
Issue number10
Pages (from-to)779-794
Number of pages16
ISSN0903-4641
DOIs
Publication statusPublished - Oct 2018

    Research areas

  • Animals, Bacteria/ultrastructure, Bacterial Infections/microbiology, Biofilms, Fluorescent Dyes/chemistry, Humans, Mice, Microscopy, Confocal/methods, Neutrophils/microbiology, Staining and Labeling/methods

ID: 56600290