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Rigshospitalet - a part of Copenhagen University Hospital
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The origin of extracellular DNA in bacterial biofilm infections in vivo

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  1. Editorial: The complexity of microbial biofilm research-an introduction to the third thematic issue on biofilms

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  2. Increased bactericidal activity of colistin on Pseudomonas aeruginosa biofilms in anaerobic conditions

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  1. APMIS pandemic editorial

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  2. The discovery of bacterial biofilm in patients with muscle invasive bladder cancer

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  3. The Extracellular Polysaccharide Matrix of Pseudomonas aeruginosa Biofilms Is a Determinant of Polymorphonuclear Leukocyte Responses

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  4. Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm

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Extracellular DNA (eDNA) plays an important role in both the aggregation of bacteria and in the interaction of the resulting biofilms with polymorphonuclear leukocytes (PMNs) during an inflammatory response. Here, transmission electron and confocal scanning laser microscopy were used to examine the interaction between biofilms of Pseudomonas aeruginosa and PMNs in a murine implant model and in lung tissue from chronically infected cystic fibrosis patients. PNA FISH, DNA staining, labeling of PMN DNA with a thymidine analogue and immunohistochemistry were applied to localize bacteria, eDNA, PMN-derived eDNA, PMN-derived histone H3 (H3), neutrophil elastase (NE) and citrullinated H3 (citH3). Host-derived eDNA was observed surrounding bacterial biofilms but not within the biofilms. H3 localized to the lining of biofilms while NE was found throughout biofilms. CitH3, a marker for neutrophil extracellular traps (NETs) was detected only sporadically indicating that most host-derived eDNA in vivo was not a result of NETosis. Together these observations show that, in these in vivo biofilm infections with P. aeruginosa, the majority of eDNA is found external to the biofilm and derives from the host.

Original languageEnglish
JournalPathogens and Disease
Volume78
Issue number2
ISSN2049-632X
DOIs
Publication statusPublished - 1 Mar 2020

    Research areas

  • Animals, Biofilms, DNA, Bacterial/metabolism, Extracellular Traps/metabolism, Histones/metabolism, Humans, Mice, Neutrophils/physiology, Pseudomonas Infections/immunology, Pseudomonas aeruginosa/physiology

ID: 62330687