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Repeated introduction and spread of the MRSA clone t304/ST6 in Northern Europe

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Bartels, MD, Worning, P, Andersen, LP, Bès, M, Enger, H, Ås, CG, Hansen, TA, Holzknecht, BJ, Larssen, KW, Laurent, F, Mäkitalo, B, Pichon, B, Svartström, O & Westh, H 2020, 'Repeated introduction and spread of the MRSA clone t304/ST6 in Northern Europe' Clinical Microbiology and Infection. https://doi.org/10.1016/j.cmi.2020.05.004

APA

CBE

Bartels MD, Worning P, Andersen LP, Bès M, Enger H, Ås CG, Hansen TA, Holzknecht BJ, Larssen KW, Laurent F, Mäkitalo B, Pichon B, Svartström O, Westh H. 2020. Repeated introduction and spread of the MRSA clone t304/ST6 in Northern Europe. Clinical Microbiology and Infection. https://doi.org/10.1016/j.cmi.2020.05.004

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Author

Bartels, Mette Damkjær ; Worning, Peder ; Andersen, Leif Percival ; Bès, Michèle ; Enger, Hege ; Ås, Christina Gabrielsen ; Hansen, Thomas Arn ; Holzknecht, Barbara Juliane ; Larssen, Kjersti Wik ; Laurent, Frederic ; Mäkitalo, Barbro ; Pichon, Bruno ; Svartström, Olov ; Westh, Henrik. / Repeated introduction and spread of the MRSA clone t304/ST6 in Northern Europe. In: Clinical Microbiology and Infection. 2020.

Bibtex

@article{488f37726530469fab6317887cbe161c,
title = "Repeated introduction and spread of the MRSA clone t304/ST6 in Northern Europe",
abstract = "OBJECTIVES: During the last decades several methicillin-resistant Staphylococcus aureus (MRSA) clones with the capability of global spread have emerged in the community. Here, we have investigated a large collection of clinical isolates belonging to MRSA clone t304/ST6, which has emerged in many European countries over the last years, in order to retrace its phylogeny and its spread.METHODS: We characterized 466 ST6 isolates from Denmark (n = 354), France (n = 10), Norway (n = 24), Sweden (n = 27) and the UK (n = 51). All had spa-type t304 (n = 454) or t304-related spa-types (n = 12) and whole genome sequencing (WGS) was carried out on Illumina Miseq or Hiseq with 100-300 bp reads. cgMLST was performed using Ridom SeqSphere.RESULTS: A minimum spanning tree (MST) of all 466 isolates showed one large cluster including 182 isolates collected only from Denmark and related to a long-term neonatal outbreak in Copenhagen. This cluster contrasted with numerous small clusters, including the remaining Danish isolates and isolates from the other countries that interspersed throughout the tree. Most isolates were Panton-Valentine leukocidin (PVL) negative (95{\%}) and harboured SCCmec IVa. One genome was closed using Oxford Nanopore technology and Illumina MiSeq. It contained a plasmid of 19.769 bp including the blaZ gene. A similar plasmid was found in 78{\%} of all isolates.DISCUSSION: t304/ST6 is a successful emerging clone and the fact that isolates from five countries are interspersed throughout the MST indicates a common origin. This clone is commonly described in the Middle East and its emergence in Europe coincides with influx of refugees from the Syrian Civil War.",
keywords = "cgMLST, MRSA, Plasmid, ST6, WGS",
author = "Bartels, {Mette Damkj{\ae}r} and Peder Worning and Andersen, {Leif Percival} and Mich{\`e}le B{\`e}s and Hege Enger and {\AA}s, {Christina Gabrielsen} and Hansen, {Thomas Arn} and Holzknecht, {Barbara Juliane} and Larssen, {Kjersti Wik} and Frederic Laurent and Barbro M{\"a}kitalo and Bruno Pichon and Olov Svartstr{\"o}m and Henrik Westh",
note = "Copyright {\circledC} 2020 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.",
year = "2020",
month = "5",
day = "18",
doi = "10.1016/j.cmi.2020.05.004",
language = "English",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Wiley-Blackwell Publishing Ltd",

}

RIS

TY - JOUR

T1 - Repeated introduction and spread of the MRSA clone t304/ST6 in Northern Europe

AU - Bartels, Mette Damkjær

AU - Worning, Peder

AU - Andersen, Leif Percival

AU - Bès, Michèle

AU - Enger, Hege

AU - Ås, Christina Gabrielsen

AU - Hansen, Thomas Arn

AU - Holzknecht, Barbara Juliane

AU - Larssen, Kjersti Wik

AU - Laurent, Frederic

AU - Mäkitalo, Barbro

AU - Pichon, Bruno

AU - Svartström, Olov

AU - Westh, Henrik

N1 - Copyright © 2020 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

PY - 2020/5/18

Y1 - 2020/5/18

N2 - OBJECTIVES: During the last decades several methicillin-resistant Staphylococcus aureus (MRSA) clones with the capability of global spread have emerged in the community. Here, we have investigated a large collection of clinical isolates belonging to MRSA clone t304/ST6, which has emerged in many European countries over the last years, in order to retrace its phylogeny and its spread.METHODS: We characterized 466 ST6 isolates from Denmark (n = 354), France (n = 10), Norway (n = 24), Sweden (n = 27) and the UK (n = 51). All had spa-type t304 (n = 454) or t304-related spa-types (n = 12) and whole genome sequencing (WGS) was carried out on Illumina Miseq or Hiseq with 100-300 bp reads. cgMLST was performed using Ridom SeqSphere.RESULTS: A minimum spanning tree (MST) of all 466 isolates showed one large cluster including 182 isolates collected only from Denmark and related to a long-term neonatal outbreak in Copenhagen. This cluster contrasted with numerous small clusters, including the remaining Danish isolates and isolates from the other countries that interspersed throughout the tree. Most isolates were Panton-Valentine leukocidin (PVL) negative (95%) and harboured SCCmec IVa. One genome was closed using Oxford Nanopore technology and Illumina MiSeq. It contained a plasmid of 19.769 bp including the blaZ gene. A similar plasmid was found in 78% of all isolates.DISCUSSION: t304/ST6 is a successful emerging clone and the fact that isolates from five countries are interspersed throughout the MST indicates a common origin. This clone is commonly described in the Middle East and its emergence in Europe coincides with influx of refugees from the Syrian Civil War.

AB - OBJECTIVES: During the last decades several methicillin-resistant Staphylococcus aureus (MRSA) clones with the capability of global spread have emerged in the community. Here, we have investigated a large collection of clinical isolates belonging to MRSA clone t304/ST6, which has emerged in many European countries over the last years, in order to retrace its phylogeny and its spread.METHODS: We characterized 466 ST6 isolates from Denmark (n = 354), France (n = 10), Norway (n = 24), Sweden (n = 27) and the UK (n = 51). All had spa-type t304 (n = 454) or t304-related spa-types (n = 12) and whole genome sequencing (WGS) was carried out on Illumina Miseq or Hiseq with 100-300 bp reads. cgMLST was performed using Ridom SeqSphere.RESULTS: A minimum spanning tree (MST) of all 466 isolates showed one large cluster including 182 isolates collected only from Denmark and related to a long-term neonatal outbreak in Copenhagen. This cluster contrasted with numerous small clusters, including the remaining Danish isolates and isolates from the other countries that interspersed throughout the tree. Most isolates were Panton-Valentine leukocidin (PVL) negative (95%) and harboured SCCmec IVa. One genome was closed using Oxford Nanopore technology and Illumina MiSeq. It contained a plasmid of 19.769 bp including the blaZ gene. A similar plasmid was found in 78% of all isolates.DISCUSSION: t304/ST6 is a successful emerging clone and the fact that isolates from five countries are interspersed throughout the MST indicates a common origin. This clone is commonly described in the Middle East and its emergence in Europe coincides with influx of refugees from the Syrian Civil War.

KW - cgMLST

KW - MRSA

KW - Plasmid

KW - ST6

KW - WGS

U2 - 10.1016/j.cmi.2020.05.004

DO - 10.1016/j.cmi.2020.05.004

M3 - Journal article

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

ER -

ID: 59931010