Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Pituitary porcine FSH, and recombinant bovine and human FSH differentially affect growth and relative abundances of mRNA transcripts of preantral and early developing antral follicles in goats

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Noncoding RNA (ncRNA) Profile Association with Patient Outcome in Epithelial Ovarian Cancer Cases

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Adverse trends in male reproductive health and decreasing fertility rates

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Use of cryopreserved ovarian tissue in the Danish fertility preservation cohort

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Effect of sphingosine-1-phosphate on activation of dormant follicles in murine and human ovarian tissue

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Anna Clara A Ferreira
  • Naiza A R Sá
  • Jesús Cadenas
  • Hudson H V Correia
  • Denise D Guerreiro
  • Benner G Alves
  • Laritza F Lima
  • Juliana J H Celestino
  • Ana Paula P R Rodrigues
  • Eduardo L Gastal
  • Jose R Figueiredo
View graph of relations

Three different sources of FSH (porcine pituitary, pFSH; recombinant bovine, rbFSH; and recombinant human, rhFSH) were compared during in vitro culture of preantral and early antral follicles of goats for 18 days. Treatments were: base medium supplemented with no FSH (control), 10, 50, or 100 mIU/mL pFSH (pFSH10, pFSH50, and pFSH100, respectively), 100 ng/mL rbFSH (rbFSH), and 50 mIU/mL rhFSH (rhFSH). There were evaluations of follicle morphology, antrum formation, growth rate, estradiol production, oocyte viability and chromatin configuration, and follicle wall relative abundance of mRNA transcript for MMP-9, TIMP-2, CYP17, CYP19A1, FSHR, Insulin-R, and BAX/BCL-2 ratio. Follicle degeneration rates were similar among all treatment groups at the end of culturing. When there were treatments with pFSH, however, there was a lesser (P < 0.05) percentage of intact follicles and estradiol production, and greater (P < 0.05) extrusion rates. Furthermore, with only pFSH10 (antral follicles) and pFSH100 (preantral and antral follicles) treatments, there was a lesser (P < 0.05) follicle growth. For preantral follicles, when there was addition of pFSH10, pFSH100, and rhFSH there was lesser (P < 0.05) oocyte meiotic resumption compared to control and rbFSH treatments. For antral follicles, when there were treatments with rhFSH and pFSH10 there was greater (P = 0.08 - P < 0.05) oocyte maturation. In conclusion, the source of FSH differentially affected gene expression, as indicated by mRNA abundances, and follicular dynamics of preantral and antral follicles in vitro. Addition of FSH during the in vitro culture improved the developmental outcomes only for antral follicles.

Original languageEnglish
Article number106461
JournalAnimal Reproduction Science
Volume219
Pages (from-to)106461
ISSN0378-4320
DOIs
Publication statusPublished - Aug 2020

    Research areas

  • Antral follicles, FSH sources, Gene expression, Goat, Oocyte maturation, Preantral follicles

ID: 61255567