Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Photic stimulation of the suprachiasmatic nucleus via the non-visual optic system. A gene expression study in the blind Crx-/- mouse

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Distribution and characterisation of CCK containing enteroendocrine cells of the mouse small and large intestine

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Analysis of enteroendocrine cell populations in the human colon

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Circadian oscillators in the mouse brain: molecular clock components in the neocortex and cerebellar cortex

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Expression of insulin signalling components in the sensory epithelium of the human saccule

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Long-term outcomes of dexamethasone 12 mg versus 6 mg in patients with COVID-19 and severe hypoxaemia

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Development of a core outcome set for general intensive care unit patients - a protocol

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Early postoperative complications were common in non-ambulant paediatric patients with neuromuscular scoliosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Management of acute atrial fibrillation in the intensive care unit: An international survey

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The visual system of vertebrates consists of an image-forming and a non-image-forming optic system; the image-forming optic system involves the classic photoreceptors, the rods and cones, whereas the non-image-forming optic system involves the melanopsin-containing retinal ganglion cells. Both optic systems make direct neuroanatomical connections to the suprachiasmatic nucleus (SCN) in the hypothalamus in which the biological clock of vertebrates is located. The rhythmic output from SCN neurons is entrained by light via the retina and the retinohypothalamic tract. The response of exposure to light during the subjective night is an immediate expression of several early response genes in the SCN. We show, by quantitative real-time polymerase chain reaction, that the amount of melanopsin mRNA in the retinal ganglion cells is preserved in the blind Crx(-/-) mouse with degenerated classic photoreceptors. At zeitgeber time 16, the Crx(-/-) and wild-type mice were exposed to 1 h of light. This resulted in a strong up-regulation of the immediate early genes Nr4a1, Erg, and Rrad in the SCN of both genotypes. Light stimulation during the subjective night resulted in a strong up-regulation of c-fos in both genotypes with a significantly higher up-regulation in the blind Crx(-/-) mouse. Expression of Grp and Vip, the genes for two classic peptides located in the SCN, was not influenced by light stimulation. The data strongly indicate the involvement of the melanopsin-based non-visual optic system in the regulation of immediate early genes in the SCN.

Original languageEnglish
JournalCell and Tissue Research
Volume358
Issue number1
Pages (from-to)239-48
Number of pages10
ISSN0302-766X
DOIs
Publication statusPublished - Oct 2014

ID: 45024453