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Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection

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Harvard

Calanchini, M, Tadman, M, Krogh, J, Fabbri, A, Grossman, A & Shine, B 2019, 'Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection' Endocrine Connections, vol. 8, no. 8, pp. 1082-1088. https://doi.org/10.1530/EC-19-0269

APA

Calanchini, M., Tadman, M., Krogh, J., Fabbri, A., Grossman, A., & Shine, B. (2019). Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection. Endocrine Connections, 8(8), 1082-1088. https://doi.org/10.1530/EC-19-0269

CBE

MLA

Vancouver

Calanchini M, Tadman M, Krogh J, Fabbri A, Grossman A, Shine B. Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection. Endocrine Connections. 2019 Aug 1;8(8):1082-1088. https://doi.org/10.1530/EC-19-0269

Author

Calanchini, Matilde ; Tadman, Michael ; Krogh, Jesper ; Fabbri, Andrea ; Grossman, Ashley ; Shine, Brian. / Measurement of urinary 5-HIAA : correlation between spot versus 24-h urine collection. In: Endocrine Connections. 2019 ; Vol. 8, No. 8. pp. 1082-1088.

Bibtex

@article{386faadfe0494a9d84f9df619e7ec03e,
title = "Measurement of urinary 5-HIAA: correlation between spot versus 24-h urine collection",
abstract = "Background: The 24-h urinary output of 5-hydroxyindoleacetic acid (5-HIAA) is used to monitor disease progression and treatment responses of neuroendocrine neoplasms (NENs). Several conditions are required for 5-HIAA assay, involving urine collection/preservation and food/drug restrictions.Aim: To evaluate the correlation between 5-HIAA concentration in a spot urine sample and the output in a 24-h urine collection, and whether spot urine specimens can replace 24-h collection.Methods: Patients with NENs or symptoms suggestive of NENs were asked to provide a separate spot urine at the end of the 24-h urine collection for 5-HIAA assessment. The upper reference limit for 24-h urinary 5-HIAA was 40 µmol/24 h. 5-HIAA measurements in spot urine samples were corrected for variation in urine flow rate by expressing results as a ratio to creatinine concentration.Results: We included 136 paired urinary samples for 5-HIAA assessment from 111 patients (100 NENs). The correlation between 5-HIAA values measured in 24-h and spot urines was r = +0.863 (P < 0.001) and r = +0.840 (P < 0.001) including only NEN patients. Using the 24-h urinary 5-HIAA as reference method, the AUC on ROC analysis for spot urinary 5-HIAA was 0.948 (95{\%} CI, 0.914-0.983; P < 0.001), attaining a sensitivity of 83{\%} and specificity of 95{\%} using 5.3 mol/mmol as cut-off for the spot urine. The AUC among NEN patients alone was 0.945 (95{\%} CI, 0.904-0.987; P < 0.001).Conclusions: The ratio of 5-HIAA to creatinine in a spot urine could replace the measurement of 5-HIAA output in a 24-h urine collection, especially for follow-up of patients with known elevated 5-HIAA levels.",
author = "Matilde Calanchini and Michael Tadman and Jesper Krogh and Andrea Fabbri and Ashley Grossman and Brian Shine",
year = "2019",
month = "8",
day = "1",
doi = "10.1530/EC-19-0269",
language = "English",
volume = "8",
pages = "1082--1088",
journal = "Endocrine Connections",
issn = "2049-3614",
publisher = "BioScientifica Ltd",
number = "8",

}

RIS

TY - JOUR

T1 - Measurement of urinary 5-HIAA

T2 - correlation between spot versus 24-h urine collection

AU - Calanchini, Matilde

AU - Tadman, Michael

AU - Krogh, Jesper

AU - Fabbri, Andrea

AU - Grossman, Ashley

AU - Shine, Brian

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Background: The 24-h urinary output of 5-hydroxyindoleacetic acid (5-HIAA) is used to monitor disease progression and treatment responses of neuroendocrine neoplasms (NENs). Several conditions are required for 5-HIAA assay, involving urine collection/preservation and food/drug restrictions.Aim: To evaluate the correlation between 5-HIAA concentration in a spot urine sample and the output in a 24-h urine collection, and whether spot urine specimens can replace 24-h collection.Methods: Patients with NENs or symptoms suggestive of NENs were asked to provide a separate spot urine at the end of the 24-h urine collection for 5-HIAA assessment. The upper reference limit for 24-h urinary 5-HIAA was 40 µmol/24 h. 5-HIAA measurements in spot urine samples were corrected for variation in urine flow rate by expressing results as a ratio to creatinine concentration.Results: We included 136 paired urinary samples for 5-HIAA assessment from 111 patients (100 NENs). The correlation between 5-HIAA values measured in 24-h and spot urines was r = +0.863 (P < 0.001) and r = +0.840 (P < 0.001) including only NEN patients. Using the 24-h urinary 5-HIAA as reference method, the AUC on ROC analysis for spot urinary 5-HIAA was 0.948 (95% CI, 0.914-0.983; P < 0.001), attaining a sensitivity of 83% and specificity of 95% using 5.3 mol/mmol as cut-off for the spot urine. The AUC among NEN patients alone was 0.945 (95% CI, 0.904-0.987; P < 0.001).Conclusions: The ratio of 5-HIAA to creatinine in a spot urine could replace the measurement of 5-HIAA output in a 24-h urine collection, especially for follow-up of patients with known elevated 5-HIAA levels.

AB - Background: The 24-h urinary output of 5-hydroxyindoleacetic acid (5-HIAA) is used to monitor disease progression and treatment responses of neuroendocrine neoplasms (NENs). Several conditions are required for 5-HIAA assay, involving urine collection/preservation and food/drug restrictions.Aim: To evaluate the correlation between 5-HIAA concentration in a spot urine sample and the output in a 24-h urine collection, and whether spot urine specimens can replace 24-h collection.Methods: Patients with NENs or symptoms suggestive of NENs were asked to provide a separate spot urine at the end of the 24-h urine collection for 5-HIAA assessment. The upper reference limit for 24-h urinary 5-HIAA was 40 µmol/24 h. 5-HIAA measurements in spot urine samples were corrected for variation in urine flow rate by expressing results as a ratio to creatinine concentration.Results: We included 136 paired urinary samples for 5-HIAA assessment from 111 patients (100 NENs). The correlation between 5-HIAA values measured in 24-h and spot urines was r = +0.863 (P < 0.001) and r = +0.840 (P < 0.001) including only NEN patients. Using the 24-h urinary 5-HIAA as reference method, the AUC on ROC analysis for spot urinary 5-HIAA was 0.948 (95% CI, 0.914-0.983; P < 0.001), attaining a sensitivity of 83% and specificity of 95% using 5.3 mol/mmol as cut-off for the spot urine. The AUC among NEN patients alone was 0.945 (95% CI, 0.904-0.987; P < 0.001).Conclusions: The ratio of 5-HIAA to creatinine in a spot urine could replace the measurement of 5-HIAA output in a 24-h urine collection, especially for follow-up of patients with known elevated 5-HIAA levels.

U2 - 10.1530/EC-19-0269

DO - 10.1530/EC-19-0269

M3 - Journal article

VL - 8

SP - 1082

EP - 1088

JO - Endocrine Connections

JF - Endocrine Connections

SN - 2049-3614

IS - 8

ER -

ID: 59064198