Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Leukocyte TNFR1 and TNFR2 Expression Contributes to the Peripheral Immune Response in Cases with Ischemic Stroke

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Interpretable Autoencoders Trained on Single Cell Sequencing Data Can Transfer Directly to Data from Unseen Tissues

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Functional Proteomic Profiling of Triple-Negative Breast Cancer

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The Vascular Circadian Clock in Chronic Kidney Disease

    Research output: Contribution to journalReviewResearchpeer-review

  4. Antimyostatin Treatment in Health and Disease: The Story of Great Expectations and Limited Success

    Research output: Contribution to journalReviewResearchpeer-review

  5. The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Socioeconomic position and maintenance therapy in children with acute lymphoblastic leukemia: A national cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. National Implementation of Simulator Training Improves Transurethral Resection of Bladder Tumours in Patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. DNA-thioguanine concentration and relapse risk in children and young adults with acute lymphoblastic leukemia: an IPD meta-analysis

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Increments in DNA-thioguanine level during thiopurine enhanced maintenance therapy of acute lymphoblastic leukemia

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Rikke B. Hansen
  • Cathrine C.H. Laursen
  • Niala Nawaz
  • Jonna S. Madsen
  • Helle H. Nielsen
  • Christina Kruuse
  • Arne Møller
  • Matilda Degn
  • Kate L. Lambertsen
View graph of relations

Tumor necrosis factor receptor 1 and 2 (TNFR1 and TNFR2) have been found in brain parenchyma of stroke patients, and plasma levels are increased in the acute phase of stroke. We evaluated associations between TNFR1 and TNFR2 plasma levels and stroke severity, infarct size, and functional outcome. Furthermore, we examined cellular expression of TNFR1 and TNFR2 on leukocyte subpopulations to explore the origin of the increased receptor levels. Blood samples were taken from 33 acute ischemic stroke patients and 10 healthy controls. TNFR1 and TNFR2 plasma concentrations were measured and correlated against the Scandinavian Stroke Scale at admission, infarct volume, and the modified Rankin Scale score three months after stroke onset. Classical, intermediate, and non-classical monocytes as well as neutrophils were purified, and cellular expression of TNFR1 and TNFR2 was examined using flow cytometry. TNFR1 and TNFR2 plasma levels were both increased after ischemic stroke, but we found no correlation with patient outcome measurements. Compared to healthy controls, ischemic stroke patients had decreased non-classical monocyte and neutrophil populations expressing TNFR1 and increased neutrophils expressing TNFR2, and decreased non-classical populations co-expressing both TNFR1 and TNFR2. This study supports the hypothesis of an acute immunological response orchestrated by the peripheral immune system following an ischemic stroke. However, the origin of the increased TNFR1 and TNFR2 plasma levels could not be clearly linked to peripheral monocytes or neutrophils. Future studies are needed and will help clarify the potential role as treatment target.

Original languageEnglish
Article number861
JournalCells
Volume10
Issue number4
ISSN2073-4409
DOIs
Publication statusPublished - 9 Apr 2021

    Research areas

  • Aged, Case-Control Studies, Female, Glial Fibrillary Acidic Protein/blood, Humans, Immunity, Intercellular Signaling Peptides and Proteins/blood, Interleukin-6/blood, Ischemic Stroke/blood, Leukocyte Count, Leukocytes/metabolism, Male, Middle Aged, Monocytes/metabolism, Neurofilament Proteins/blood, Neutrophils/metabolism, Receptors, CCR2/metabolism, Receptors, Tumor Necrosis Factor, Type I/blood, Receptors, Tumor Necrosis Factor, Type II/blood, Severity of Illness Index, Stroke/blood, Treatment Outcome

ID: 66269910