Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

In-vivo gentamicin susceptibility test for prevention of bacterial biofilms in bone tissue and on implants

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Implications of the EUCAST Trailing Phenomenon in Candida tropicalis for the In Vivo Susceptibility in Invertebrate and Murine Models

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. APX001A In Vitro Activity against Contemporary Blood Isolates and Candida auris Determined by the EUCAST Reference Method

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Population Pharmacokinetics of the Antimalarial Amodiaquine: a Pooled Analysis To Optimize Dosing

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. EUCAST Determination of Olorofim (F901318) Susceptibility of Mold Species, Method Validation, and MICs

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. UV light assisted antibiotics for eradication of in vitro biofilms

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Biofilm formation - what we can learn from recent developments

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The use of fluorescent staining techniques for microscopic investigation of polymorphonuclear leukocytes and bacteria

    Research output: Contribution to journalReviewResearchpeer-review

  4. Bacterial biofilm formation inside colonic crypts may accelerate colorectal carcinogenesis

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Implants induce a new niche for microbiomes

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Louise Kruse Jensen
  • Thomas Bjarnsholt
  • Kasper N Kragh
  • Bent Aalbæk
  • Nicole Lind Henriksen
  • Sophie Amalie Blirup
  • Karen Pankoke
  • Andreas Petersen
  • Henrik Elvang Jensen
View graph of relations

OBJECTIVES: To set up an in-vivo gentamicin susceptibility test for biofilm prevention in bone tissue and on implants.

METHODS: Twenty-five pigs were allocated to six groups. Group A (n=6) was inoculated with saline. Groups B (n=6), C (n=3), D (n=3), E (n=3) and F (n=4) were inoculated with 10 μL saline containing 104 CFU of Staphylococcus aureus Different concentrations based on the minimal inhibitory concentrations (MIC) of gentamicin to the specific strain were added to the 10 μL inoculum of Groups C (160xMIC), D (1,600xMIC), E (16,000xMIC) and F (160,000xMIC). The inoculums were injected into a pre-drilled tibial implant cavity followed by insertion of a steel implant (2 × 15 mm). The pigs were euthanized after five days. In-vitro, all the used doses were found bacteriostatic after up to 6 hours.

RESULTS: All implant cavities of pigs inoculated with bacteria and bacteria + 160xMIC or 1,600xMIC were positive for S. aureus In each of the Groups E (16,000xMIC) and F (160,000xMIC) 2/3 and 1/4 of the implant cavities were S. aureus positive, respectively. By grouping Groups C + D (<10,000xMIC) and Groups E + F (>10,000xMIC) a significant decrease of implant attached bacteria was only seen between the high MIC value group and Group B. Histologically, it was demonstrated that 1,600, 16,000 and 160,000 x MIC resulted in a peri-implant tissue reaction comparable to saline inoculated animals.

CONCLUSION: In-vivo, the antimicrobial tolerance of the inoculated planktonic bacteria was increased by in-vivo specific factors of acute inflammation. This resulted in bacterial aggregation and biofilm formation which further increased the gentamicin tolerance. Thus, susceptibility patterns in-vitro might not reflect the actual in-vivo susceptibility locally within a developing infectious area.

Original languageEnglish
JournalAntimicrobial Agents and Chemotherapy
Volume63
Issue number2
Pages (from-to)e01889-18
ISSN0066-4804
DOIs
Publication statusPublished - 2019

ID: 56132363