Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
E-pub ahead of print

Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Approach to the patient with glucocorticoid-induced adrenal insufficiency

    Research output: Contribution to journalReviewResearchpeer-review

  2. Quinine effects on gut and pancreatic hormones and antropyloroduodenal pressures in humans-role of delivery site and sex

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Dynamic changes of reproductive hormones in male minipuberty: Temporal dissociation of Leydig- and Sertoli-cell activity

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Patients with cirrhosis have elevated bone turnover but normal hepatic production of osteoprotegerin

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Lipoprotein(a) levels at birth and in early childhood: The COMPARE Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Characterization and Survival of Human Infant Testicular Cells After Direct Xenotransplantation

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Reintroducing serum FSH measurement during ovarian stimulation for ART

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Fertility preservation in boys facing gonadotoxic cancer therapy

    Research output: Contribution to journalReviewResearchpeer-review

  4. Expression and Role of INSL3 in the Fetal Testis

    Research output: Contribution to journalReviewResearchpeer-review

View graph of relations

CONTEXT: The oocyte-secreted factors GDF9 and BMP15 play essential roles in follicle development and oocyte maturation, and aberrant regulation might contribute to the pathogenesis of PCOS.

OBJECTIVE: Are there measurable differences in concentrations of GDF9, BMP15, and the GDF9/BMP15 heterodimer in small antral follicle fluids from women with and without polycystic ovaries (PCO)?

DESIGN & SETTING: Follicle fluids (n=356) were collected from 4-11 mm follicles in unstimulated ovaries of 87 women undergoing ovarian tissue cryopreservation for fertility preservation.

PATIENTS: Twenty-seven women with PCO were identified and 60 women without PCO-like characteristics (non-PCO women) were matched according to age and follicle size.

MAIN OUTCOME MEASURES: Intrafollicular concentrations of GDF9, BMP15, GDF9/BMP15 heterodimer, AMH, inhibin-A and -B, total inhibin, activin-B and -AB, and follistatin were measured using ELISA-assays.

RESULTS: The detectability of GDF9, BMP15, and the GDF9/BMP15 heterodimer were 100%, 94.4%, and 91.5% respectively, and concentrations were significantly negatively correlated with increasing follicle size (P<0.0001). GDF9 was significantly higher in women with PCO (PCO: 4230±189 pg/ml (mean±SEM), n=188; non-PCO: 3498±199 pg/ml, n=168; P<0.03), whereas BMP15 was lower in women with PCO (PCO: 431±40 pg/ml, n=125; non-PCO: 573±55 pg/ml, n=109; P=0.10), leading to a significantly higher GDF9:BMP15 ratio in women with PCO (P<0.01). Significant positive associations between BMP15 and AMH, activins, and inhibins in non-PCO women switched to negative associations in women with PCO.

CONCLUSIONS: Intrafollicular concentrations of GDF9 and BMP15 varied inversely in women with PCO reflecting an aberrant endocrine environment. An increased GDF9:BMP15 ratio may be a new biomarker for PCO.

Original languageEnglish
JournalThe Journal of clinical endocrinology and metabolism
ISSN0021-972X
DOIs
Publication statusE-pub ahead of print - 2 May 2022

Bibliographical note

© The Author(s) 2022. Published by Oxford University Press on behalf of the Endocrine Society.

    Research areas

  • PCOS, follicle fluid, antral follicle, BMP15, GDF9, cumulin

ID: 77928488