Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

In vivo imaging of cell proliferation in meningioma using 3'-deoxy-3'-[18F]fluorothymidine PET/MRI

Research output: Contribution to journalJournal articleResearchpeer-review

  1. COVID-19 and the brain: impact on nuclear medicine in neurology

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Pharmacokinetic analysis of [68Ga]Ga-DOTA-TOC PET in meningiomas for assessment of in vivo somatostatin receptor subtype 2

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. High Sympathetic Activity in Raynaud's Disease

    Research output: Contribution to journalConference articleResearchpeer-review

  4. EANM practice guideline/SNMMI procedure standard for dopaminergic imaging in Parkinsonian syndromes 1.0

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Cerebrovascular effects of glibenclamide investigated using high-resolution magnetic resonance imaging in healthy volunteers

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Non-invasive assessment of temporal changes in myocardial microvascular function in persons with type 2 diabetes and healthy controls

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Prevalence of cognitive impairment and its relation to mental health in Danish lymphoma survivors

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Past, present and future, the experience of time during examination for malignant brain tumor: a qualitative observational study

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

PURPOSE: Positron emission tomography (PET) with 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT) provides a noninvasive assessment of tumour proliferation in vivo and could be a valuable imaging modality for assessing malignancy in meningiomas. We investigated a range of static and dynamic [18F]FLT metrics by correlating the findings with cellular biomarkers of proliferation and angiogenesis.

METHODS: Seventeen prospectively recruited adult patients with intracranial meningiomas underwent a 60-min dynamic [18F]FLT PET following surgery. Maximum and mean standardized uptake values (SUVmax, SUVmean) with and without normalization to healthy brain tissue and blood radioactivity obtained from 40 to 60 min summed dynamic images (PET40-60) and ~ 60-min blood samples were calculated. Kinetic modelling using a two-tissue reversible compartmental model with a fractioned blood volume (VB) was performed to determine the total distribution volume (VT). Expressions of proliferation and angiogenesis with key parameters including Ki-67 index, phosphohistone-H3 (phh3), MKI67, thymidine kinase 1 (TK1), proliferating cell nuclear antigen (PCNA), Kirsten RAt Sarcoma viral oncogene homolog (KRAS), TIMP metallopeptidase inhibitor 3 (TIMP3), and vascular endothelial growth factor A (VEGFA) were determined by immunohistochemistry and/or quantitative polymerase chain reaction.

RESULTS: Immunohistochemistry revealed 13 World Health Organization (WHO) grade I and four WHO grade II meningiomas. SUVmax and SUVmean normalized to blood radioactivity from PET40-60 and blood sampling, and VT were able to significantly differentiate between WHO grades with the best results for maximum and mean tumour-to-whole-blood ratios (sensitivity 100%, specificity 94-95%, accuracy 99%; P = 0.003). Static [18F]FLT metrics were significantly correlated with proliferative biomarkers, especially Ki-67 index, phh3, and TK1, while no correlations were found with VEGFA or VB. Using Ki-67 index with a threshold > 4%, the majority of [18F]FLT metrics showed a high ability to identify aggressive meningiomas with SUVmean demonstrating the best performance (sensitivity 80%, specificity 81%, accuracy 80%; P = 0.024).

CONCLUSION: [18F]FLT PET could be a useful imaging modality for assessing cellular proliferation in meningiomas.

Original languageEnglish
JournalEuropean Journal of Nuclear Medicine and Molecular Imaging
Volume47
Issue number6
Pages (from-to)1496-1509
Number of pages14
ISSN1619-7070
DOIs
Publication statusPublished - Jun 2020

    Research areas

  • Ki-67 index, Meningioma, Thymidine kinase 1, [F-18]FLT

ID: 61372652