Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Improved assessment of outcomes following transient global cerebral ischemia in mice

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Short-term cortical plasticity induced by conditioning pain modulation

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The effect of baclofen and diazepam on motor skill acquisition in healthy subjects

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Cortical involvement in anticipatory postural reactions in man

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Cerebral ischemia induces transcription of inflammatory and extracellular-matrix-related genes in rat cerebral arteries

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Ovariectomy reduces vasocontractile responses of rat middle cerebral arteries after focal cerebral ischemia

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Hormonal influences in migraine - interactions of oestrogen, oxytocin and CGRP

    Research output: Contribution to journalReviewResearchpeer-review

  3. CGRP and migraine; from bench to bedside

    Research output: Contribution to journalReviewResearchpeer-review

View graph of relations

Mouse models of global cerebral ischemia (GCI) allow experimental examination of cerebral pathophysiology in genetically modified mice and fast screening of new treatment strategies. Various surgical protocols of GCI-induction in mice have been published; however, many of these studies are hindered by limited neurological assessment protocols and present insufficient reporting of the cumulative survival rate. Therefore, we aim at developing a reproducible and easily implementable model of transient GCI in mice with minimal impact on normal mouse behavior. GCI was induced in male C57BL/6 mice by bilateral occlusion of the common carotid arteries for 10 min combined with isoflurane-induced hypotension which resulted in severe reduction in the cerebral blood flow of the forebrain. Sham operation served as a control. Exploratory behavior was evaluated in a home-cage environment the day before and again daily for up to 7 days after GCI or sham operation and was found to be significantly decreased 1-7 days after GCI compared to sham. Furthermore, we found delayed neuronal cell death in the frontal cortex and hippocampus 5 and 7 days after GCI but not at day 3 or after sham operation. The survival rate at day 7 was 100 % after sham operation and 42 % after GCI. The model of GCI in mice presented in this study compromises the exploratory behavior and resembles the cerebral damage and mortality rate seen after cardiac arrest and/or GCI in man, and is therefore a good model to use for studies of GCI pathophysiology.

Original languageEnglish
JournalExperimental Brain Research
Volume234
Issue number7
Pages (from-to)1925-34
ISSN0014-4819
DOIs
Publication statusPublished - Jul 2016

ID: 46389822