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Rigshospitalet - a part of Copenhagen University Hospital
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IgG avidity to Pseudomonas aeruginosa over the course of chronic lung biofilm infection in cystic fibrosis

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  1. Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients

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  2. Markers of bone turnover are reduced in patients with CF related diabetes; the role of glucose

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  3. Seasonal fluctuation of lung function in cystic fibrosis: A national register-based study in two northern European populations

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  4. Antibody response against Mycobacterium avium complex in cystic fibrosis patients measured by a novel IgG ELISA test

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  1. Primary ciliary dyskinesia patients have the same P. aeruginosa clone in sinuses and lungs

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  2. Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients

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  3. Antibiotic therapy as personalized medicine - general considerations and complicating factors

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  4. Editorial comment

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BACKGROUND AND OBJECTIVES: The mechanisms leading to low effectiveness of the humoral immune response against P. aeruginosa in cystic fibrosis (CF) are poorly understood. The aim of the present study was to assess the avidity maturation of specific antipseudomonal IgG before and during the development of chronic lung infection in a cohort of Danish CF patients.

METHODS: Avidity maturation was assessed against a pooled P. aeruginosa antigen (St-Ag) and against P. aeruginosa alginate in 10 CF patients who developed chronic lung infection and 10 patients who developed intermittent lung colonization, using an ELISA technique with the thiocyanate elution method. Avidity was quantitatively determined by calculating the avidity Constant (Kav).

RESULTS: IgG avidity to St-Ag significantly increased at the onset (Median Kav=2.47) and one year after the onset of chronic infection (Median Kav=3.27), but did not significantly changed in patients who developed intermittent colonization. IgG avidity against alginate did not significantly change over the years neither in patients who developed chronic lung infection (Median Kav=3.84 at the onset of chronic infection), nor in patients who developed intermittent colonization.

CONCLUSION: IgG avidity to P. aeruginosa alginate does not significantly enhance as chronic lung infection progresses. This probably plays a role in the difficulty to mount an effective opsonophagocytic killing to clear mucoid P. aeruginosa infection in CF.

Original languageEnglish
JournalJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
Volume17
Issue number3
Pages (from-to)356-359
Number of pages4
ISSN1569-1993
DOIs
Publication statusPublished - May 2018

ID: 56586665