Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Identification of Novel Native Autoantigens in Rheumatoid Arthritis

Research output: Contribution to journalJournal articleResearchpeer-review

  1. The Importance of Lipoprotein Lipase Regulation in Atherosclerosis

    Research output: Contribution to journalReviewResearchpeer-review

  2. Risk of Chronic Obstructive Pulmonary Disease Exacerbation in Patients Who Use Methotrexate-A Nationwide Study of 58,580 Outpatients

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. GLP-1 and Intestinal Diseases

    Research output: Contribution to journalReviewResearchpeer-review

  1. Effectiveness of brodalumab after previous treatment failure of interleukin-17A inhibitors in patients with psoriasis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Acute and persistent symptoms in non-hospitalized PCR-confirmed COVID-19 patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T)

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Elevated levels of oxidized nucleosides in individuals with the JAK2V617F mutation from a general population study

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Complement split product C3c in saliva as biomarker for periodontitis and response to periodontal treatment

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The majority of patients diagnosed with rheumatoid arthritis (RA) have developed autoantibodies against neoepitopes in proteins that have undergone post-translational modification, e.g., citrullination or carbamylation. There is growing evidence of their molecular relevance and their potential utility to improve diagnosis, patient stratification, and prognosis for precision medicine. Autoantibodies reacting to native proteins may also have a role in RA pathogenesis, however, their reactivity patterns remain much less studied. We hypothesized that a high-density protein array technology could shed light onto the normal and disease-related autoantibodies produced in healthy and RA patient subgroups. In an exploratory study, we investigated the global reactivity of autoantibodies in plasma pools from 15 anti-cyclic citrullinated peptide (CCP)-positive and 10 anti-CCP-negative RA patients and 10 healthy donors against more than 1600 native and unmodified human proteins using a high-density protein array. A total of 102 proteins recognized by IgG autoantibodies were identified, hereof 86 were recognized by antibodies from CCP-positive RA patients and 76 from anti-CCP-negative RA patients, but not by antibodies from healthy donors. Twenty-four of the identified autoantigens have previously been identified in synovial fluid. Multiple human proteins in their native conformation are recognized by autoantibodies from anti-CCP-positive as well as anti-CCP-negative RA patients.

Original languageEnglish
JournalBiomedicines
Volume8
Issue number6
Pages (from-to)141
Number of pages13
ISSN2227-9059
DOIs
Publication statusPublished - Jun 2020

    Research areas

  • protein array, rheumatoid arthritis, autoantibodies, native autoantigens, immunome, microarray

ID: 61865012