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Rigshospitalet - a part of Copenhagen University Hospital
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Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients

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  1. The impact of chest computed tomography and chest radiography on clinical management of cystic fibrosis lung disease

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  2. Amikacin liposome inhalation suspension for chronic Pseudomonas aeruginosa infection in cystic fibrosis

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  3. Antisense oligonucleotide eluforsen is safe and improves respiratory symptoms in F508DEL cystic fibrosis

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  4. Markers of bone turnover are reduced in patients with CF related diabetes; the role of glucose

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  1. The efficacy of topical agents used in wounds for managing chronic biofilm infections: A systematic review

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  2. The environmental occurrence of Pseudomonas aeruginosa

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  3. Biofilms of Mycobacterium abscessus complex can be sensitized to antibiotics by disaggregation and oxygenation

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  4. Minimum information guideline for spectrofotometric and fluorometric methods to assess biofilm formation in microplates

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BACKGROUND: Pseudomonas aeruginosa is a major pathogen of the chronic lung infections in cystic fibrosis (CF) patients. These persistent bacterial infections are characterized by bacterial aggregates with biofilm-like properties and are treated with nebulized or intravenous tobramycin in combination with other antibiotics. However, the chronic infections are close to impossible to eradicate due to reasons that are far from fully understood. Recent work has shown that re‑oxygenation of hypoxic aggregates by hyperbaric oxygen (O2) treatment (HBOT: 100% O2 at 2.8 bar) will increase killing of aggregating bacteria by antibiotics. This is relevant for treatment of infected CF patients where bacterial aggregates are found in the endobronchial secretions that are depleted of O2 by the metabolism of polymorphonuclear leukocytes (PMNs). The main objective of this study was to investigate the effect of HBOT as an adjuvant to tobramycin treatment of aggregates formed by P. aeruginosa isolates from CF patients.

METHODS: The effect was tested using a model with bacterial aggregates embedded in agarose. O2 profiling was used to confirm re‑oxygenation of aggregates.

RESULTS: We found that HBOT was able to significantly enhance the effect of tobramycin against aggregates of all the P. aeruginosa isolates in vitro. The effect was attributed to increased O2 levels leading to increased growth and thus increased uptake of and killing by tobramycin.

CONCLUSIONS: Re‑oxygenation may in the future be a clinical possibility as adjuvant to enhance killing by antibiotics in cystic fibrosis lung infections.

Original languageEnglish
JournalJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
Volume18
Issue number5
Pages (from-to)657-664
Number of pages8
ISSN1569-1993
DOIs
Publication statusPublished - Sep 2019

ID: 58995108