Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Gestational age-dependent development of the neonatal metabolome

Research output: Contribution to journalJournal articleResearchpeer-review

  1. European consensus recommendations for neonatal and paediatric retrievals of positive or suspected COVID-19 patients

    Research output: Contribution to journalReviewResearchpeer-review

  2. Changes in pulmonary oxygen content are detectable with laser absorption spectroscopy: proof of concept in newborn piglets

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Gut transit time, using radiological contrast imaging, to predict early signs of necrotizing enterocolitis

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Does height and IGF-I determine pubertal timing in girls?

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Increased antioxidant response in medium-chain acyl-CoA dehydrogenase deficiency: does lipoic acid have a protective role?

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. The Role of Leptin in Fetal Growth during Pre-Eclampsia

    Research output: Contribution to journalReviewResearchpeer-review

  2. Danish premature birth rates during the COVID-19 lockdown

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Low-grade inflammation independently associates with cardiometabolic risk in children with overweight/obesity

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Leptin, adiponectin, and their ratio as markers of insulin resistance and cardiometabolic risk in childhood obesity

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Madeleine Ernst
  • Simon Rogers
  • Ulrik Lausten-Thomsen
  • Anders Björkbom
  • Susan Svane Laursen
  • Julie Courraud
  • Anders Børglum
  • Merete Nordentoft
  • Thomas Werge
  • Preben Bo Mortensen
  • David M Hougaard
  • Arieh S Cohen
View graph of relations

BACKGROUND: Prematurity is a severe pathophysiological condition, however, little is known about the gestational age-dependent development of the neonatal metabolome.

METHODS: Using an untargeted liquid chromatography-tandem mass spectrometry metabolomics protocol, we measured over 9000 metabolites in 298 neonatal residual heel prick dried blood spots retrieved from the Danish Neonatal Screening Biobank. By combining multiple state-of-the-art metabolome mining tools, we retrieved chemical structural information at a broad level for over 5000 (60%) metabolites and assessed their relation to gestational age.

RESULTS: A total of 1459 (~16%) metabolites were significantly correlated with gestational age (false discovery rate-adjusted P < 0.05), whereas 83 metabolites explained on average 48% of the variance in gestational age. Using a custom algorithm based on hypergeometric testing, we identified compound classes (617 metabolites) overrepresented with metabolites correlating with gestational age (P < 0.05). Metabolites significantly related to gestational age included bile acids, carnitines, polyamines, amino acid-derived compounds, nucleotides, phosphatidylcholines and dipeptides, as well as treatment-related metabolites, such as antibiotics and caffeine.

CONCLUSIONS: Our findings elucidate the gestational age-dependent development of the neonatal blood metabolome and suggest that the application of metabolomics tools has great potential to reveal novel biochemical underpinnings of disease and improve our understanding of complex pathophysiological mechanisms underlying prematurity-associated disorders.

IMPACT: A large variation in the neonatal dried blood spot metabolome from residual heel pricks stored at the Danish Neonatal Screening Biobank can be explained by gestational age. While previous studies have assessed the relation of selected metabolic markers to gestational age, this study assesses metabolome-wide changes related to prematurity. Using a combination of recently developed metabolome mining tools, we assess the relation of over 9000 metabolic features to gestational age. The ability to assess metabolome-wide changes related to prematurity in neonates could pave the way to finding novel biochemical underpinnings of health complications related to preterm birth.

Original languageEnglish
JournalPediatric Research
Volume89
Issue number6
Pages (from-to)1396-1404
Number of pages9
ISSN0031-3998
DOIs
Publication statusPublished - May 2021

ID: 61716472