Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Gene Expression in Granulosa Cells From Small Antral Follicles From Women With or Without Polycystic Ovaries

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Increases in bioactive IGF do not parallel increases in total IGF-I during growth hormone treatment of children born SGA

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Muscle-liver substrate fluxes in exercising humans and potential effects on hepatic metabolism

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. A Polygenic Risk Score Suggests Shared Genetic Architecture of Voice Break With Early Markers of Pubertal Onset in Boys

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Effect of dosage of 17ß-estradiol on uterine growth in Turner syndrome - a randomized controlled clinical pilot trial

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Gestational Diabetes Risk in Migrants. A Nationwide, Register-Based Study of all Births in Denmark 2004 to 2015

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Ovarian cortical follicle density in infertile women with low anti-Müllerian hormone

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

CONTEXT: Polycystic ovary syndrome (PCOS) is the most common cause of anovulation. A key feature of PCOS is arrest of follicles at the small- to medium-sized antral stage.

OBJECTIVE AND DESIGN: To provide further insight into the mechanism of follicle arrest in PCOS, we profiled (i) gonadotropin receptors; (ii) characteristics of aberrant steroidogenesis; and (iii) expression of anti-Müllerian hormone (AMH) and its receptor in granulosa cells (GCs) from unstimulated, human small antral follicles (hSAFs) and from granulosa lutein cells (GLCs).

SETTING: GCs from hSAFs were collected at the time of cryopreservation of ovarian tissue for fertility preservation and GLCs collected during oocyte aspiration before in vitro fertilization/intracytoplasmic sperm injection.

PARTICIPANTS: We collected hSAF GCs from 31 women (98 follicles): 10 with polycystic ovaries (PCO) and 21 without. GLCs were collected from 6 women with PCOS and 6 controls undergoing IVF.

MAIN OUTCOME MEASURES: Expression of the following genes: LHCGR, FSHR, AR, INSR, HSD3B2, CYP11A1, CYP19, STAR, AMH, AMHR2, FST, INHBA, INHBB in GCs and GLCs were compared between women with PCO and controls.

RESULTS: GCs in hSAFs from women with PCO showed higher expression of LHCGR in a subset (20%) of follicles. Expression of FSHR (P < 0.05), AR (P < 0.05), and CYP11A1 (P < 0.05) was lower, and expression of CYP19A1 (P < 0.05), STAR (P < 0.05), HSD3B2 (P = NS), and INHBA (P < 0.05) was higher in PCO GCs. Gene expression in GL cells differed between women with and without PCOS but also differed from that in GCs.

CONCLUSIONS: Follicle arrest in PCO is characterized in GCs by differential regulation of key genes involved in follicle growth and function.

Original languageEnglish
JournalThe Journal of clinical endocrinology and metabolism
Volume104
Issue number12
Pages (from-to)6182-6192
Number of pages11
ISSN0021-972X
DOIs
Publication statusPublished - 1 Dec 2019

ID: 59045258