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Rigshospitalet - a part of Copenhagen University Hospital
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Gel-based proteomics of liver cancer progression in rat

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  1. Proteomics of differential extraction fractions enriched for chromatin-binding proteins from colon adenoma and carcinoma tissues

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  2. Calcium electroporation in three cell lines: a comparison of bleomycin and calcium, calcium compounds, and pulsing conditions

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  3. Tumor interstitial fluid - a treasure trove of cancer biomarkers

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  4. Apolipoprotein M promotes mobilization of cellular cholesterol in vivo

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  5. The interplay between SUCLA2, SUCLG2, and mitochondrial DNA depletion

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  1. Evaluation of Serum Insulin-like Factor 3 Quantification by LC-MS/MS as a Biomarker of Leydig Cell Function

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  2. Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LC-MS/MS

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  3. Terror på hjernen

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  4. Clinical proteomics: Insights from IGF-I

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  5. Development and validation of a mass spectrometry-based assay for quantification of insulin-like factor 3 in human serum

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A significant challenge in proteomics biomarker research is to identify the changes that are of highest diagnostic interest, among the many unspecific aberrations associated with disease burden and inflammation. In the present study liver tissue specimens (n=18) from six experimental stages were collected from the resistant hepatocyte (RH) rat model of liver cancer and analyzed by 2D DIGE. The study included triplicates of regenerating liver, control "sham-operated" liver, three distinct premalignant stages and hepatomas. Out of 81 identified proteins two-thirds were differentially abundant in rat hepatomas compared to control rat liver and, secondly, the majority of proteins were also changed in precursor stages. This underscores the importance of adequate control samples in explorative cancer biomarker research. We confirm several proteomic changes previously identified in human hepatocellular carcinoma (HCC) and we identify novel candidate proteomic aberrations for further analysis in human HCC. In particular, increased levels of HSP70, HSP90, AKR1B1, AKR7A3, GCLM, ANXA5, VDBP, RGN and SULT1E1 were associated specifically with rat hepatomas, or with liver cancer progression in rat. In addition, we examine an integrated gel-based workflow for analysis of protein post-translational modifications (PTMs) and microtubule-association. We highlight differential PTM and localization of HSP60 as an interesting target for further analysis in liver cancer.

Original languageEnglish
JournalBiochimica et Biophysica Acta
Volume1814
Issue number10
Pages (from-to)1367-76
Number of pages10
ISSN0006-3002
DOIs
Publication statusPublished - Oct 2011

    Research areas

  • Animals, Carcinoma, Hepatocellular, Disease Models, Animal, Disease Progression, Humans, Liver Neoplasms, Male, Microtubule-Associated Proteins, Phosphoproteins, Proteomics, Rats, Rats, Inbred F344, Two-Dimensional Difference Gel Electrophoresis, Journal Article, Research Support, Non-U.S. Gov't

ID: 49496816