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Rigshospitalet - a part of Copenhagen University Hospital
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Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling

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Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.

Original languageEnglish
JournalBioanalysis
Volume12
Issue number10
Pages (from-to)665-681
Number of pages17
ISSN1757-6180
DOIs
Publication statusPublished - 1 May 2020

    Research areas

  • cortisol, dried blood spot, fluispotter, hematocrit, LC–MS/MS, microsampling

ID: 59978293