Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Filamentous bacteriophages are associated with chronic Pseudomonas lung infections and antibiotic resistance in cystic fibrosis

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Imaging-assisted nanoimmunotherapy for atherosclerosis in multiple species

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Enhanced detection of circulating tumor DNA by fragment size analysis

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Evaluation of liquid from the Papanicolaou test and other liquid biopsies for the detection of endometrial and ovarian cancers

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. A glucagon-like peptide-1 receptor agonist reduces intracranial pressure in a rat model of hydrocephalus

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Radiocarbon dating reveals minimal collagen turnover in both healthy and osteoarthritic human cartilage

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Primary ciliary dyskinesia patients have the same P. aeruginosa clone in sinuses and lungs

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Variant in ERAP1 promoter region is associated with low expression in a patient with a Behçet-like MHC-I-opathy

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Structure of Pseudomonas aeruginosa ribosomes from an aminoglycoside-resistant clinical isolate

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Filamentous bacteriophage (Pf phage) contribute to the virulence of Pseudomonas aeruginosa infections in animal models, but their relevance to human disease is unclear. We sought to interrogate the prevalence and clinical relevance of Pf phage in patients with cystic fibrosis (CF) using sputum samples from two well-characterized patient cohorts. Bacterial genomic analysis in a Danish longitudinal cohort of 34 patients with CF revealed that 26.5% (n = 9) were consistently Pf phage positive. In the second cohort, a prospective cross-sectional cohort of 58 patients with CF at Stanford, sputum qPCR analysis showed that 36.2% (n = 21) of patients were Pf phage positive. In both cohorts, patients positive for Pf phage were older, and in the Stanford CF cohort, patients positive for Pf phage were more likely to have chronic P. aeruginosa infection and had greater declines in pulmonary function during exacerbations than patients negative for Pf phage presence in the sputum. Last, P. aeruginosa strains carrying Pf phage exhibited increased resistance to antipseudomonal antibiotics. Mechanistically, in vitro analysis showed that Pf phage sequesters these same antibiotics, suggesting that this mechanism may thereby contribute to the selection of antibiotic resistance over time. These data provide evidence that Pf phage may contribute to clinical outcomes in P. aeruginosa infection in CF.

Original languageEnglish
JournalScience translational medicine
Volume11
Issue number488
Pages (from-to)eaau9748
ISSN1946-6234
DOIs
Publication statusPublished - 17 Apr 2019

ID: 59017738