Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

ERG Controls B Cell Development by Promoting Igh V-to-DJ Recombination

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Single mRNP Analysis Reveals that Small Cytoplasmic mRNP Granules Represent mRNA Singletons

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Gamma-Aminobutyric Acid Signaling in Brown Adipose Tissue Promotes Systemic Metabolic Derangement in Obesity

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Single mRNP Analysis Reveals that Small Cytoplasmic mRNP Granules Represent mRNA Singletons

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Heterozygous loss of Srp72 in mice is not associated with major hematological phenotypes

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Deconvolution of autoencoders to learn biological regulatory modules from single cell mRNA sequencing data

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. A Gain-of-Function p53-Mutant Oncogene Promotes Cell Fate Plasticity and Myeloid Leukemia through the Pluripotency Factor FOXH1

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

B cell development depends on the coordinated expression and cooperation of several transcription factors. Here we show that the transcription factor ETS-related gene (ERG) is crucial for normal B cell development and that its deletion results in a substantial loss of bone marrow B cell progenitors and peripheral B cells, as well as a skewing of splenic B cell populations. We find that ERG-deficient B lineage cells exhibit an early developmental block at the pre-B cell stage and proliferate less. The cells fail to express the immunoglobulin heavy chain due to inefficient V-to-DJ recombination, and cells that undergo recombination display a strong bias against incorporation of distal V gene segments. Furthermore, antisense transcription at PAX5-activated intergenic repeat (PAIR) elements, located in the distal region of the Igh locus, depends on ERG. These findings show that ERG serves as a critical regulator of B cell development by ensuring efficient and balanced V-to-DJ recombination.

Original languageEnglish
JournalCell Reports
Volume29
Issue number9
Pages (from-to)2756-2769.e6
DOIs
Publication statusPublished - 26 Nov 2019

    Research areas

  • B cell development, ERG, ETS-related gene, immunoglobulin heavy-chain gene, pre-BCR, transcriptional control, V(D)J recombination

ID: 58519830