Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Differences in global DNA methylation of testicular seminoma are not associated with changes in histone modifications, clinical prognosis, BRAF mutations or gene expression

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Genomic diagnostics leading to the identification of a TFG-ROS1 fusion in a child with possible atypical meningioma

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. A new NFIA:RAF1 fusion activating the MAPK pathway in pilocytic astrocytoma

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Heterogeneity of chromatin modifications in testicular spermatocytic seminoma point toward an epigenetically unstable phenotype

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Breast and Prostate Cancer Risks for Male BRCA1 and BRCA2 Pathogenic Variant Carriers Using Polygenic Risk Scores

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Paternity After Treatment for Testicular Germ Cell Cancer: A Danish Nationwide Population-Based Cohort Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Novel Genetic Causes of Gastrointestinal Polyposis Syndromes

    Research output: Contribution to journalReviewResearchpeer-review

  4. Calcium transport in male reproduction is possibly influenced by vitamin D and CaSR

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Testicular germ cell tumours of young adults are comprised of a heterogeneous group of non-seminomas and a homogeneous group of seminomas. While the majority of seminomas retain a hypo-methylated genome, a small fraction displays a highly methylated genome, resembling hyper-methylated non-seminomas. It is well established from e.g. melanoma, colorectal and thyroid cancer that a methylated phenotype can be correlated to prognosis and can be related to BRAF mutations. In the present study we investigated the global methylation level in 67 seminomas and classified them as hypo-methylated, intermediate, patchy and hyper-methylated, respectively. A selected subset representing each level of DNA methylation and the TCam2 seminoma cell line were subsequently analysed for a range of other epigenetic marks (6 histone marks and 5-hydroxymethylcytosine), the presence of the BRAF V600E de novo mutation, differences in the transcriptome and finally correlated to the clinical outcome. We did not identify any histone marks or hydroxymethylation levels that correlated with the methylation level of the genome. Some histone marks, however, showed a great variation while others were found at the same level in all the investigated seminomas. We did not identify any tumours with the BRAF V600E mutation and transcriptome analysis revealed no significant differences between hypo- and hyper-methylated seminomas. Similarly, no obvious differences in the clinical manifestation of the patients representing hypo- or hyper-methylated seminomas were identified. The level of DNA methylation in testicular seminomas consequently seems secondary to the manifestation of the tumour phenotype.

Original languageEnglish
JournalCancer genetics
Volume209
Issue number11
Pages (from-to)506-514
Number of pages9
ISSN2210-7762
DOIs
Publication statusPublished - Nov 2016

ID: 49789842