Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Cerebrovascular Gene Expression in Spontaneously Hypertensive Rats After Transient Middle Cerebral Artery Occlusion

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Transcranial Alternating Current Stimulation Has Frequency-Dependent Effects on Motor Learning in Healthy Humans

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Functional Neuroimaging in Disorders of Consciousness: Raising Awareness for Those with Decreased Awareness

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Perinatal nicotine treatment induces transient increases in NACHO protein levels in the rat frontal cortex

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Whole transcriptome expression of trigeminal ganglia compared to dorsal root ganglia in Rattus Norvegicus

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Oral rimegepant for migraine prevention

    Research output: Contribution to journalComment/debateResearchpeer-review

  2. Estrogen receptors α, β and GPER in the CNS and trigeminal system - molecular and functional aspects

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Hypertension is a major risk factor for stroke, which is one of the leading global causes of death. In the search for new and effective therapeutic targets in stroke research, we need to understand the influence of hypertension in the vasculature following stroke. We used Affymetrix whole-transcriptome expression profiling as a tool to address gene expression differences between the occluded and non-occluded middle cerebral arteries (MCAs) from spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats after transient middle cerebral artery occlusion (tMCAO), to provide clues about the pathological mechanisms set in play after stroke. Verified by quantitative PCR, expression of Ccl2, Edn1, Tgfβ2, Olr1 and Serpine1 was significantly increased in the occluded compared to non-occluded MCAs from both SHRs and WKY rats. Additionally, expression of Mmp9, Icam1, Hif1α and Timp1 was increased in the occluded compared to non-occluded MCAs isolated from WKY rats. In comparison between occluded MCAs from SHRs versus occluded MCAs from WKY rats, expression of Ccl2, Olr1 and Serpine1 was significantly increased in SHR MCAs. However, the opposite was observed regarding expression of Edn1. Thus these data suggest that Ccl2, Edn1, Tgfβ2, Olr1 and Serpine1 may be possible mediators of the vascular changes in the occluded MCAs from both SHRs and WKY rats after tMCAO. The aforementioned genes possess biological functions that are consistent with early stroke injuries. In conclusion, these genes may be potential targets in future strategies for acute stroke treatments that can be used in patients with and without hypertension.

Original languageEnglish
JournalNeuroscience
Volume367
Pages (from-to)219-232
Number of pages14
ISSN0306-4522
DOIs
Publication statusPublished - 2017

    Research areas

  • Journal Article

ID: 52342131