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Rigshospitalet - a part of Copenhagen University Hospital
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CDH1 (E-cadherin) in testicular germ cell neoplasia: suppressed translation of mRNA in pre-invasive carcinoma in situ but increased protein levels in advanced tumours

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  1. Primary antibiotic resistance of Helicobacter pylori strains among adults and children in a tertiary referral centre in Lithuania

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  2. A short history of microbial biofilms and biofilm infections

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  3. Biofilms and host response - helpful or harmful

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  4. Diagnosis of biofilm infections in cystic fibrosis patients

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  1. Age-related changes in human Leydig cell status

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  2. Associations of the gut microbiome and clinical factors with acute GVHD in allogeneic HSCT recipients

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  3. Rucaparib in Men With Metastatic Castration-Resistant Prostate Cancer Harboring a BRCA1 or BRCA2 Gene Alteration

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  4. Impact of psychological stress measured in three different scales on testis function: A cross-sectional study of 1362 young men

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E-cadherin (CDH1) is a transmembrane glycoprotein involved in cellular adhesion. In our recent microarray studies of testicular germ cell tumours (TGCTs) and the common precursor carcinoma in situ (CIS), CDH1 mRNA was highly expressed in CIS and embryonal carcinoma. It has previously been reported that the CDH1 protein is not expressed in CIS. To resolve the discrepancy, we performed a detailed analysis of the expression of CDH1 mRNA and protein in a series of normal and neoplastic testes. High expression of CDH1 mRNA in CIS was confirmed by real-time PCR and in situ hybridisation. At the protein level, however, CDH1 was only detected with one of three tested antibodies, but Western blotting analysis with this antibody showed additional bands, suggesting unspecific staining. The levels of a CDH1 protein fragment in serum samples from 58 patients with TGCTs were analysed by ELISA; we found significantly higher levels in patients with advanced disease (stage II/III) when compared to healthy individuals and patients with stage I TGCT. In conclusion, despite high mRNA levels, the CDH1 protein is not expressed in CIS, suggesting translational suppression of CDH1 protein expression. CDH1 serum levels may be a serological marker for staging of TGCT patients.
Original languageEnglish
JournalAPMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Volume114
Issue number7-8
Pages (from-to)549-58
Number of pages10
DOIs
Publication statusPublished - 2006

ID: 31067430