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Calcitonin gene-related peptide and its receptor components in the human sphenopalatine ganglion -- interaction with the sensory system

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@article{26211d0966834bf288136b9066aff99b,
title = "Calcitonin gene-related peptide and its receptor components in the human sphenopalatine ganglion -- interaction with the sensory system",
abstract = "Clinical studies have suggested a link between the sensory trigeminal system and the parasympathetic ganglia. Calcitonin gene-related peptide (CGRP) is a sensory neuropeptide which plays an important role in vasodilatation and pain transmission in craniocervical structures. The present study was designed to examine if CGRP and CGRP receptor components are present in the human sphenopalatine ganglion (SPG) in order to reveal an interaction between the sensory and parasympathetic systems. Indirect immunofluorescence technique was used for immunohistochemical demonstration of CGRP, the calcitonin receptor-like receptor (CLR) and the receptor activity modifying protein 1 (RAMP1) in human and rat SPG. Cryostat sections were examined and images were obtained using a light- and epifluorescence microscope coupled to a camera to visualize co-labeling by superimposing the digital images. In addition, Western blot technique was used to demonstrate the existence of CGRP receptor components in rat SPG. CGRP immunoreactive fibers were frequently found intraganglionic in the SPG in the vicinity of neurons. CLR immunoreactivity was observed in satellite glial cells (SGCs) as well as in nerve fibers, but not in neurons. RAMP1 immunoreactivity was localized in many neurons and SGCs. Thus, the two CGRP receptor components together were found in the SGCs. In addition, Western blot revealed the presence of RAMP1 and CLR in rat SPG. Our results suggest a possible sensory influence in the parasympathetic cranial ganglia. The sensory CGRP-containing fibers probably originate in the trigeminal ganglion, project to the SPG and act on CGRP receptors on SGCs.",
keywords = "Aged, Aged, 80 and over, Animals, Calcitonin Gene-Related Peptide, Female, Humans, Male, Middle Aged, Neuroglia, Neurons, Rats, Rats, Sprague-Dawley, Receptor Activity-Modifying Protein 1, Receptors, Calcitonin Gene-Related Peptide, Trigeminal Ganglion",
author = "Anett Csati and Janos Tajti and Bernadett Tuka and Lars Edvinsson and Karin Warfvinge",
note = "Copyright {\textcopyright} 2011 Elsevier B.V. All rights reserved.",
year = "2012",
doi = "10.1016/j.brainres.2011.11.058",
language = "English",
volume = "1435",
pages = "29--39",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Calcitonin gene-related peptide and its receptor components in the human sphenopalatine ganglion -- interaction with the sensory system

AU - Csati, Anett

AU - Tajti, Janos

AU - Tuka, Bernadett

AU - Edvinsson, Lars

AU - Warfvinge, Karin

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Clinical studies have suggested a link between the sensory trigeminal system and the parasympathetic ganglia. Calcitonin gene-related peptide (CGRP) is a sensory neuropeptide which plays an important role in vasodilatation and pain transmission in craniocervical structures. The present study was designed to examine if CGRP and CGRP receptor components are present in the human sphenopalatine ganglion (SPG) in order to reveal an interaction between the sensory and parasympathetic systems. Indirect immunofluorescence technique was used for immunohistochemical demonstration of CGRP, the calcitonin receptor-like receptor (CLR) and the receptor activity modifying protein 1 (RAMP1) in human and rat SPG. Cryostat sections were examined and images were obtained using a light- and epifluorescence microscope coupled to a camera to visualize co-labeling by superimposing the digital images. In addition, Western blot technique was used to demonstrate the existence of CGRP receptor components in rat SPG. CGRP immunoreactive fibers were frequently found intraganglionic in the SPG in the vicinity of neurons. CLR immunoreactivity was observed in satellite glial cells (SGCs) as well as in nerve fibers, but not in neurons. RAMP1 immunoreactivity was localized in many neurons and SGCs. Thus, the two CGRP receptor components together were found in the SGCs. In addition, Western blot revealed the presence of RAMP1 and CLR in rat SPG. Our results suggest a possible sensory influence in the parasympathetic cranial ganglia. The sensory CGRP-containing fibers probably originate in the trigeminal ganglion, project to the SPG and act on CGRP receptors on SGCs.

AB - Clinical studies have suggested a link between the sensory trigeminal system and the parasympathetic ganglia. Calcitonin gene-related peptide (CGRP) is a sensory neuropeptide which plays an important role in vasodilatation and pain transmission in craniocervical structures. The present study was designed to examine if CGRP and CGRP receptor components are present in the human sphenopalatine ganglion (SPG) in order to reveal an interaction between the sensory and parasympathetic systems. Indirect immunofluorescence technique was used for immunohistochemical demonstration of CGRP, the calcitonin receptor-like receptor (CLR) and the receptor activity modifying protein 1 (RAMP1) in human and rat SPG. Cryostat sections were examined and images were obtained using a light- and epifluorescence microscope coupled to a camera to visualize co-labeling by superimposing the digital images. In addition, Western blot technique was used to demonstrate the existence of CGRP receptor components in rat SPG. CGRP immunoreactive fibers were frequently found intraganglionic in the SPG in the vicinity of neurons. CLR immunoreactivity was observed in satellite glial cells (SGCs) as well as in nerve fibers, but not in neurons. RAMP1 immunoreactivity was localized in many neurons and SGCs. Thus, the two CGRP receptor components together were found in the SGCs. In addition, Western blot revealed the presence of RAMP1 and CLR in rat SPG. Our results suggest a possible sensory influence in the parasympathetic cranial ganglia. The sensory CGRP-containing fibers probably originate in the trigeminal ganglion, project to the SPG and act on CGRP receptors on SGCs.

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Calcitonin Gene-Related Peptide

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Neuroglia

KW - Neurons

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptor Activity-Modifying Protein 1

KW - Receptors, Calcitonin Gene-Related Peptide

KW - Trigeminal Ganglion

U2 - 10.1016/j.brainres.2011.11.058

DO - 10.1016/j.brainres.2011.11.058

M3 - Journal article

C2 - 22208649

VL - 1435

SP - 29

EP - 39

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -

ID: 36859117