Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

Blood group genotyping of blood donors: validation of a highly accurate routine method

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Smoking and risk of surgical bleeding: nationwide analysis of 5,452,411 surgical cases

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The donors perceived positive and negative effects of blood donation

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Can text messages save lives? A field experiment on blood donor motivation

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Oral iron supplementation is not associated with short-term risk of infections: results from the Danish Blood Donor Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Frequent blood donation and offspring scholastic attainment: an assessment of long-term consequences of prenatal iron deficiency

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Treatment of monogenic disorders with viral transduced haematopoietic stem cells

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Impact of long-term storage of plasma and cell-free DNA on measured DNA quantity and fetal fraction

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Erythrocytapheresis as a novel treatment option for adult patients with pyruvate kinase deficiency

    Research output: Contribution to journalEditorialpeer-review

  4. External quality assessment of noninvasive fetal RHD genotyping

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

BACKGROUND: In the past century, blood group determination using serology has been the standard method. Now, molecular methods are gaining traction, which provide additional and easily accessible information. Here we designed and validated a high-throughput extended genotyping setup.

STUDY DESIGN AND METHODS: We developed 35 competitive allele-specific polymerase chain reaction assays for genotyping of blood donors. Samples from 1034 Danish blood donors were genotyped, and 45,314 red blood cell antigens and 6148 platelet antigens were predicted. Predicted phenotypes were compared with 16,119 serologic phenotypes.

RESULTS: We found 62 discrepancies of which 43 were due to serology. After exclusion of the discrepancies caused by serology, the accuracy of genotyping was 99.9%. Of 17 discrepancies caused by the genotype, three were incorrect antigen-negative predictions and could potentially, as the solitary analysis, have caused an adverse transfusion reaction.

CONCLUSION: We have established a robust and highly accurate blood group genotyping system with a very high capacity for screening blood donors. The system represents a significant improvement over the former serotyping-only procedure. Almost all new technology in medicine incurs increased costs, but the presented efficient genotyping system is a rare example of a significant qualitative and quantitative technologic progress that is also more cost-efficient than previous technologies.

Original languageEnglish
JournalTransfusion
Volume59
Issue number10
Pages (from-to)3264-3274
ISSN0041-1132
DOIs
Publication statusPublished - 15 Aug 2019

Bibliographical note

Publisher Copyright:
© 2019 AABB

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

ID: 57849730