Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital

BDNF downregulates 5-HT(2A) receptor protein levels in hippocampal cultures

Research output: Contribution to journalJournal articleResearchpeer-review

  1. TDP-43-specific Autoantibody Decline in Patients With Amyotrophic Lateral Sclerosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Blocking of efflux transporters in rats improves translational validation of brain radioligands

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT(2A)) have been related to depression pathology. Specific 5-HT(2A) receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT(2A) receptor level. Here we show a direct effect of BDNF on 5-HT(2A) receptor protein levels in primary hippocampal neuronal and mature hippocampal organotypic cultures exposed to different BDNF concentrations for either 1, 3, 5 or 7 days. In vivo effects of BDNF on hippocampal 5-HT(2A) receptor levels were further corroborated in (BDNF +/-) mice with reduced BDNF levels. In primary neuronal cultures, 7 days exposure to 25 and 50ng/mL BDNF resulted in downregulation of 5-HT(2A), but not of 5-HT(1A), receptor protein levels. The BDNF-associated downregulation of 5-HT(2A) receptor levels was also observed in mature hippocampal organotypic cultures, excluding confounding effects of BDNF on immature tissue. BDNF +/- mice showed significant increased 5-HT(2A) receptor levels in hippocampus confirming the association between 5-HT(2A) receptor and BDNF levels in vivo. In conclusion, our results point to a regulatory role of BDNF on 5-HT2A receptor levels. This interaction may be an important mechanism in the role of BDNF in affective disorders emphasizing the need for further elucidating the specificity and the mechanism behind this regulation.

Original languageEnglish
JournalNeurochemistry International
Issue number7
Pages (from-to)697-702
Number of pages6
Publication statusPublished - Dec 2009

    Research areas

  • Animals, Apoptosis, Blotting, Western, Brain-Derived Neurotrophic Factor, Cells, Cultured, Down-Regulation, Female, Hippocampus, Mice, Mice, Knockout, Nerve Tissue Proteins, Organ Culture Techniques, Pregnancy, Rats, Rats, Sprague-Dawley, Receptor, Serotonin, 5-HT2A, Synapses

ID: 45604349