Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

An exploratory study of microbial diversity in sinus infections of cystic fibrosis patients by molecular methods

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Seasonal fluctuation of lung function in cystic fibrosis: A national register-based study in two northern European populations

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Antibody response against Mycobacterium avium complex in cystic fibrosis patients measured by a novel IgG ELISA test

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Defining antimicrobial resistance in cystic fibrosis

    Research output: Contribution to journalReviewResearchpeer-review

  4. The choice of lung function reference equation affects clinical trial eligibility: Results from a cystic fibrosis cohort

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. IgG avidity to Pseudomonas aeruginosa over the course of chronic lung biofilm infection in cystic fibrosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Dynamic soft tissue changes in the orbit after a blowout fracture

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. PET/CT prior to salvage surgery in recurrent head and neck squamous cell carcinoma

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Reliability and validity of The Danish pediatric voice handicap index

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Acquired complement C1 esterase inhibitor deficiency in a patient with a rare SERPING1 variant with unknown significance

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

BACKGROUND: For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center.

METHODS: Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis.

RESULTS: Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes.

CONCLUSIONS: The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.

Original languageEnglish
JournalJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
Volume13
Issue number6
Pages (from-to)645-52
Number of pages8
ISSN1569-1993
DOIs
Publication statusPublished - Dec 2014

ID: 44955184