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Rigshospitalet - a part of Copenhagen University Hospital
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Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats

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  • Niels H Søe
  • Nina Vendel Jensen
  • Asger Lundorff Jensen
  • Janne Koch
  • Steen Seier Poulsen
  • Gerald B Pier
  • Helle Krogh Johansen
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BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.

MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 103S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.

RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica+) but not ica-S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica+or ica-S. aureus, suggesting a lack of specificity in this antiserum.

Original languageEnglish
JournalIn vivo (Athens, Greece)
Volume31
Issue number1
Pages (from-to)45-50
Number of pages6
ISSN0258-851X
DOIs
Publication statusPublished - 2 Jan 2017

    Research areas

  • Animals, Biomarkers, Immunization, Male, Osteomyelitis, Periprosthetic Fractures, Rabbits, Rats, Rats, Sprague-Dawley, Staphylococcal Infections, Staphylococcus aureus, Journal Article

ID: 52702009