Research
Print page Print page
Switch language
Rigshospitalet - a part of Copenhagen University Hospital
Published

A Novel Assay for Easy and Rapid Quantification of Helicobacter pylori Adhesion

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Inflammation, Immunity, and Vaccines for Helicobacter pylori Infection

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Sequence Type 410 Is Causing New International High-Risk Clones

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Traffic flow and microbial air contamination in operating rooms at a major teaching hospital in Ghana

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

BACKGROUND: Reducing adhesion of Helicobacter pylori to gastric epithelial cells could be a new way to counteract infections with this organism. We here present a novel method for quantification of Helicobacter pylori adhesion to cells.

METHODS: Helicobacter pylori is allowed to adhere to AGS or MKN45g cells in a 96-well microtiter plate. Then wells are added saponin, which lyses the cells without affecting the bacteria. After addition of alamarBlue(®) (resazurin) and 1- to 2-hour incubation, fluorescence measurements can be used to quantify the number of adherent bacteria.

RESULTS: By use of the method, we demonstrate that adhesion of both a sabA and babA deletion mutant of H. pylori is significantly reduced compared to the wild type.

CONCLUSION: The method offers a number of applications and may be used to compare the adherence potential of different strains of H. pylori to either cells or different materials or to screen for potential anti-adhesive compounds. The results presented here suggest that this easy and reproducible assay is well suited for quantitative investigation of H. pylori adhesion.

Original languageEnglish
JournalHelicobacter (Oxford, Online)
Volume20
Issue number3
Pages (from-to)199-205
Number of pages7
ISSN1523-5378
DOIs
Publication statusPublished - Jun 2015

ID: 46284662