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A Novel Assay for Easy and Rapid Quantification of Helicobacter pylori Adhesion

Research output: Contribution to journalJournal articleResearchpeer-review


  1. Inflammation, Immunity, and Vaccines for Helicobacter pylori Infection

    Research output: Contribution to journalJournal articleResearchpeer-review

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BACKGROUND: Reducing adhesion of Helicobacter pylori to gastric epithelial cells could be a new way to counteract infections with this organism. We here present a novel method for quantification of Helicobacter pylori adhesion to cells.

METHODS: Helicobacter pylori is allowed to adhere to AGS or MKN45g cells in a 96-well microtiter plate. Then wells are added saponin, which lyses the cells without affecting the bacteria. After addition of alamarBlue(®) (resazurin) and 1- to 2-hour incubation, fluorescence measurements can be used to quantify the number of adherent bacteria.

RESULTS: By use of the method, we demonstrate that adhesion of both a sabA and babA deletion mutant of H. pylori is significantly reduced compared to the wild type.

CONCLUSION: The method offers a number of applications and may be used to compare the adherence potential of different strains of H. pylori to either cells or different materials or to screen for potential anti-adhesive compounds. The results presented here suggest that this easy and reproducible assay is well suited for quantitative investigation of H. pylori adhesion.

Original languageEnglish
JournalHelicobacter (Oxford, Online)
Issue number3
Pages (from-to)199-205
Number of pages7
Publication statusPublished - Jun 2015

ID: 46284662