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Rigshospitalet - en del af Københavns Universitetshospital
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The structurally effect of surface coated rhamnogalacturonan I on response of the osteoblast-like cell line SaOS-2

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  • Rikke Svava
  • Katarzyna Gurzawska
  • Yu Yihau
  • Kenneth Brian Haugshøj
  • Kai Dirscherl
  • Steven Bruce Levery
  • Niklas Rye Jørgensen
  • Klaus Gotfredsen
  • Iben Damager
  • Peter Ulvskov
  • Bodil Jørgensen
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Osseointegration is important when implants are inserted into the bone and can be improved by biochemical surface coating of the implant. In this paper enzymatically modified rhamnogalacturonan I (RG-I) from apple and lupin was used for biochemical coating of aminated surfaces and the importance of the quality of RG-I, the nature of the binding, the fine structure of RG-I, and its effect on SaOS-2 cell line cultured on coated surfaces was investigated. SaOS-2 cells are osteoblast-like cells and a well-established in vitro model of bone-matrix forming osteoblasts. Purification by gel filtration could remove small fragments of galacturonic acid (GalA) and binding studies showed that the purity of the RG-I molecules was important for the quality of the coating. The structure of RG-I and osteoblast-like cells' viability were positively correlated so that high content of 1,4-linked galactose (Gal) and a low content of arabinose in the RG-I molecules favored cell viability. These results indicate that coating of implants with RG-I affect osseointegration positively. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

OriginalsprogEngelsk
TidsskriftJournal of Biomedical Materials Research. Part A
Vol/bind102
Udgave nummer6
Sider (fra-til)1961-1971
Antal sider11
ISSN1549-3296
DOI
StatusUdgivet - jun. 2014

ID: 43540687