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Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LC-MS/MS

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Evaluation of Serum Insulin-like Factor 3 Quantification by LC-MS/MS as a Biomarker of Leydig Cell Function

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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  4. Increases in bioactive IGF do not parallel increases in total IGF-I during growth hormone treatment of children born SGA

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  5. Muscle-liver substrate fluxes in exercising humans and potential effects on hepatic metabolism

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Evaluation of Serum Insulin-like Factor 3 Quantification by LC-MS/MS as a Biomarker of Leydig Cell Function

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Luteinizing Hormone Receptor Is Expressed in Testicular Germ Cell Tumors: Possible Implications for Tumor Growth and Prognosis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Use of stored serum in the study of time trends and geographical differences in exposure of pregnant women to phthalates

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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CONTEXT: The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations.

OBJECTIVE: To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3).

DESIGN: LC-MS/MS method development and construction of estrogen reference ranges.

SETTINGS: Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas.

PARTICIPANTS: Healthy participants aged 3 months to 61 years (n = 1838).

RESULTS: An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2, and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged from <LOD to 100 pmol/L during mini-puberty, whereas it was ≤20 pmol/L during childhood. E1 and E2 increased with age and pubertal breast stage and varied during the menstrual cycle; E1 was lower than E2 in girls and premenopausal women, and higher than E2 in postmenopausal women. In boys, E1 and E2 increased with age and pubertal stage, whereas little changes with age were observed in men. High E3 concentrations were confirmed in pregnant women.

CONCLUSION: Reference ranges of simultaneous quantification of E1 and E2 by this novel specific and highly sensitive LC-MS/MS method provide an invaluable tool in clinical practice and in future research studies.

OriginalsprogEngelsk
TidsskriftThe Journal of clinical endocrinology and metabolism
Vol/bind105
Udgave nummer3
Sider (fra-til)1-15
ISSN0021-972X
DOI
StatusUdgivet - 1 mar. 2020

Bibliografisk note

© Endocrine Society 2019.

ID: 58366501