Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
E-pub ahead of print

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LC-MS/MS

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Effect of the incretin hormones on the endocrine pancreas in end-stage renal disease

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Hydroxylated Long-Chain Acylcarnitines are Biomarkers of Mitochondrial Myopathy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Investigating Intestinal Glucagon after Roux-en-Y Gastric Bypass Surgery

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Surgical Management, Preoperative Tumor Localization, and Histopathology of 80 Patients Operated on for Insulinoma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Adipose tissue concentrations of non-persistent environmental phenols and local redox balance in adults from Southern Spain

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Copeptin, a surrogate marker for arginine vasopressin secretion, is positively associated with glucagon

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

CONTEXT: The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations.

OBJECTIVE: To establish sex specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art LC-MS/MS method for quantification of E1, E2, and estriol (E3).

DESIGN: LC-MS/MS method development and construction of estrogen reference ranges.

SETTINGS: Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas.

PARTICIPANTS: Healthy participants aged 3 months to 61 years (n=1838).

RESULTS: An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2 and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged from <LOD-100 pmol/L during mini-puberty, whereas it was ≤20 pmol/L during childhood. E1 and E2 increased with age and pubertal breast stage and varied during the menstrual cycle; E1 was lower than E2 in girls and premenopausal women, and higher than E2 in postmenopausal women. In boys, E1 and E2 increased with age and pubertal stage, whereas little changes with age were observed in men. High E3 concentrations were confirmed in pregnant women.

CONCLUSION: Reference ranges of simultaneous quantification of E1 and E2 by this novel specific and highly sensitive LC-MS/MS method provide an invaluable tool in clinical practice as well as in future research studies.

OriginalsprogEngelsk
TidsskriftThe Journal of clinical endocrinology and metabolism
ISSN0021-972X
DOI
StatusE-pub ahead of print - 13 nov. 2019

Bibliografisk note

© Endocrine Society 2019.

ID: 58366501