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Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Retinal pigment epithelial cells upregulate expression of complement factors after co-culture with activated T cells

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  • Helene Bæk Juel
  • Charlotte Kaestel
  • Lasse Westergaard Folkersen
  • Carsten Faber
  • Niels Henrik Helweg Heegaard
  • Rehannah Borup
  • Mogens Holst Nissen
Vis graf over relationer
In this study we examined the effect of T cell-derived cytokines on retinal pigment epithelial (RPE) cells with respect to expression of complement components. We used an in vitro co-culture system in which CD3/CD28-activated human T cells were separated from the human RPE cell line (ARPE-19) by a membrane. Differential gene expression in the RPE cells of complement factor genes was identified using gene arrays, and selected gene transcripts were validated by q-RT-PCR. Protein expression was determined by ELISA and immunoblotting. Co-culture with activated T cells increased RPE mRNA and/or protein expression of complement components C3, factors B, H, H-like 1, CD46, CD55, CD59, and clusterin, in a dose-dependent manner. Soluble factors derived from activated T cells are capable of increasing expression of complement components in RPE cells. This is important for the further understanding of inflammatory ocular diseases such as uveitis and age-related macular degeneration.
OriginalsprogEngelsk
TidsskriftExperimental Eye Research
Vol/bind92
Udgave nummer3
Sider (fra-til)180-8
Antal sider9
ISSN0014-4835
DOI
StatusUdgivet - 2011

ID: 33202412