Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Postnatal germ cell development in cryptorchid boys

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Fatty acid intake in relation to reproductive hormones and testicular volume among young healthy men

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Minutes of the 5th meeting of the International Network for Young Researchers in Male Fertility

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Meiotic crossover: what controls the breaks?

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Normal reference ranges for semen quality and their relations to fecundity

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Germ cells positive for PLAP and c-Kit in 11-16 year old normal boys with ongoing spermatogenesis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Expression of the Insulin-like Growth Factor System in First- and Second-Trimester Human Embryonic and Fetal Gonads

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Shortcomings of an unphysiological triggering of oocyte maturation using human chorionic gonadotropin

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  4. Richter's transformation in patients with chronic lymphocytic leukaemia: a Nationwide Epidemiological Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Surrogate testes: Allogeneic spermatogonial stem cell transplantation within an encapsulation device may restore male fertility

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Cryptorchidism is associated with infertility in adulthood. Early orchiopexy is suggested to reduce the risk. Information is lacking on the potential link between infant germ cell maturation and the risk of future infertility. The objective of the study was to evaluate age-related germ cell development in cryptorchidism. Immunostaining for markers of germ cell development (octamer-binding transcription factor 3/4 [OCT3/4], placental alkaline phosphatase [PLAP], KIT proto-oncogene [C-KIT], podoplanin [D2-40], Lin-28 homolog A [LIN28], and G antigen 7 [GAGE-7]) was performed in testicular biopsies from 40 cryptorchid boys aged 4-35 months. Germ cell numbers and distributions were evaluated in cross sections of seminiferous tubules, with and without immunostaining. OCT3/4, D2-40, and LIN28 were generally expressed in the early stages of germ cell development, as shown by positive expression in germ cells in the central region of seminiferous tubules. In contrast, PLAP and GAGE-7 were expressed in both central and peripheral parts of the tubules in the early stages of development and expressed mainly in a peripheral position with advancing age. Germ cell maturation was delayed in this study population as compared with that observed in our previous study on germ cell markers in a healthy population. The number of GAGE-7-positive germ cells per tubular cross section obtained by immunostaining was significantly higher than that obtained by standard hematoxylin and eosin staining. Double immunostaining revealed heterogeneity in germ cell development in cryptorchid testes. These results shed light on the pathophysiology of germ cell development in boys with cryptorchidism.

OriginalsprogEngelsk
TidsskriftAsian Journal of Andrology
Vol/bind22
Udgave nummer3
Sider (fra-til)258-264
Antal sider7
ISSN1008-682X
DOI
StatusUdgivet - 1 maj 2020

ID: 60626666