Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Li, Ruili ; Azzollini, Damian ; Shen, Ruidong ; Thorup, Jorgen ; Clasen-Linde, Erick ; Cortes, Dina ; Hutson, John M. / Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome. I: Journal of Pediatric Surgery. 2019 ; Bind 54, Nr. 8. s. 1654-1659.

Bibtex

@article{41e41f4376954b6b80aeaa2690f2555d,
title = "Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome",
abstract = "INTRODUCTION: Neonatal testicular germ cells/gonocytes, transform into stem cells for spermatogenesis during 'minipuberty', driving change in timing of surgery. This study examined gonocyte transformation in cryptorchid testes in children ≤18 months of age with unilateral, bilateral undescended testes (UDT), complete or partial androgen insensitivity syndrome (CAIS, PAIS) [3,4].MATERIAL AND METHODS: Testicular biopsies were taken from patients with unilateral or bilateral UDT, PAIS or CAIS, aged 10 days-18 months. These testicular sections underwent immunohistochemistry with antibodies (Oct4, Ki67, C-Kit, Sox9) followed by confocal imaging, cell counting and statistical analysis.RESULTS: Both Sertoli cells/tubule and germ cells (GC)/tubule decreased with age, and {\%} empty tubules (no GC) increased with age but with no significant differences between patient groups. Oct4+ germ cells/tubule decreased with age. There are some GCs and Sertoli cells proliferating during the first year and most proliferating Oct4+ germ cells (Oct4+/Ki67+) were located off tubular basement membrane.CONCLUSION: Our study showed that Oct4 expression gradually decreased after minipuberty and transformation into spermatogonia. Germ cells and Sertoli cells undergo mitosis during the first 12 months although not abundantly. We propose that Oct4+ gonocyte transformation into spermatogonia via proliferation and migration to the basement membrane may be delayed in UDT.",
keywords = "Cryptorchidism, Germ cells, Gonocyte, Spermatogonium, Testicular cancer, Undescended testis, Cryptorchidism/pathology, Octamer Transcription Factor-3/metabolism, Spermatogenesis, Cell Count, Humans, Infant, Male, Androgen-Insensitivity Syndrome/pathology, Sertoli Cells/pathology, Basement Membrane/pathology, Testis/pathology, Spermatogonia/metabolism, Cell Differentiation, Infant, Newborn",
author = "Ruili Li and Damian Azzollini and Ruidong Shen and Jorgen Thorup and Erick Clasen-Linde and Dina Cortes and Hutson, {John M}",
note = "Copyright {\circledC} 2018 Elsevier Inc. All rights reserved.",
year = "2019",
month = "8",
day = "1",
doi = "10.1016/j.jpedsurg.2018.12.011",
language = "English",
volume = "54",
pages = "1654--1659",
journal = "Journal of Pediatric Surgery",
issn = "0022-3468",
publisher = "W.B./Saunders Co",
number = "8",

}

RIS

TY - JOUR

T1 - Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome

AU - Li, Ruili

AU - Azzollini, Damian

AU - Shen, Ruidong

AU - Thorup, Jorgen

AU - Clasen-Linde, Erick

AU - Cortes, Dina

AU - Hutson, John M

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - INTRODUCTION: Neonatal testicular germ cells/gonocytes, transform into stem cells for spermatogenesis during 'minipuberty', driving change in timing of surgery. This study examined gonocyte transformation in cryptorchid testes in children ≤18 months of age with unilateral, bilateral undescended testes (UDT), complete or partial androgen insensitivity syndrome (CAIS, PAIS) [3,4].MATERIAL AND METHODS: Testicular biopsies were taken from patients with unilateral or bilateral UDT, PAIS or CAIS, aged 10 days-18 months. These testicular sections underwent immunohistochemistry with antibodies (Oct4, Ki67, C-Kit, Sox9) followed by confocal imaging, cell counting and statistical analysis.RESULTS: Both Sertoli cells/tubule and germ cells (GC)/tubule decreased with age, and % empty tubules (no GC) increased with age but with no significant differences between patient groups. Oct4+ germ cells/tubule decreased with age. There are some GCs and Sertoli cells proliferating during the first year and most proliferating Oct4+ germ cells (Oct4+/Ki67+) were located off tubular basement membrane.CONCLUSION: Our study showed that Oct4 expression gradually decreased after minipuberty and transformation into spermatogonia. Germ cells and Sertoli cells undergo mitosis during the first 12 months although not abundantly. We propose that Oct4+ gonocyte transformation into spermatogonia via proliferation and migration to the basement membrane may be delayed in UDT.

AB - INTRODUCTION: Neonatal testicular germ cells/gonocytes, transform into stem cells for spermatogenesis during 'minipuberty', driving change in timing of surgery. This study examined gonocyte transformation in cryptorchid testes in children ≤18 months of age with unilateral, bilateral undescended testes (UDT), complete or partial androgen insensitivity syndrome (CAIS, PAIS) [3,4].MATERIAL AND METHODS: Testicular biopsies were taken from patients with unilateral or bilateral UDT, PAIS or CAIS, aged 10 days-18 months. These testicular sections underwent immunohistochemistry with antibodies (Oct4, Ki67, C-Kit, Sox9) followed by confocal imaging, cell counting and statistical analysis.RESULTS: Both Sertoli cells/tubule and germ cells (GC)/tubule decreased with age, and % empty tubules (no GC) increased with age but with no significant differences between patient groups. Oct4+ germ cells/tubule decreased with age. There are some GCs and Sertoli cells proliferating during the first year and most proliferating Oct4+ germ cells (Oct4+/Ki67+) were located off tubular basement membrane.CONCLUSION: Our study showed that Oct4 expression gradually decreased after minipuberty and transformation into spermatogonia. Germ cells and Sertoli cells undergo mitosis during the first 12 months although not abundantly. We propose that Oct4+ gonocyte transformation into spermatogonia via proliferation and migration to the basement membrane may be delayed in UDT.

KW - Cryptorchidism

KW - Germ cells

KW - Gonocyte

KW - Spermatogonium

KW - Testicular cancer

KW - Undescended testis

KW - Cryptorchidism/pathology

KW - Octamer Transcription Factor-3/metabolism

KW - Spermatogenesis

KW - Cell Count

KW - Humans

KW - Infant

KW - Male

KW - Androgen-Insensitivity Syndrome/pathology

KW - Sertoli Cells/pathology

KW - Basement Membrane/pathology

KW - Testis/pathology

KW - Spermatogonia/metabolism

KW - Cell Differentiation

KW - Infant, Newborn

U2 - 10.1016/j.jpedsurg.2018.12.011

DO - 10.1016/j.jpedsurg.2018.12.011

M3 - Journal article

VL - 54

SP - 1654

EP - 1659

JO - Journal of Pediatric Surgery

JF - Journal of Pediatric Surgery

SN - 0022-3468

IS - 8

ER -

ID: 56518908