Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Cryptorchidism, gonocyte development, and the risks of germ cell malignancy and infertility: A systematic review

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  2. Impaired serum inhibin-B and number of germ cells in boys with cryptorchidism following heavily gestational maternal smoking

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Outcomes of biliary atresia in the Nordic countries - a multicenter study of 158 patients during 2005-2016

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Postnatal germ cell development in cryptorchid boys

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Review of injection techniques for spermatogonial stem cell transplantation

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. Laparoscopy to Assist Surgical Decisions Related to Necrotizing Enterocolitis in Preterm Neonates

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Reply by Authors

    Publikation: Bidrag til tidsskriftKommentar/debatForskning

  • Ruili Li
  • Damian Azzollini
  • Ruidong Shen
  • Jorgen Thorup
  • Erick Clasen-Linde
  • Dina Cortes
  • John M Hutson
Vis graf over relationer

INTRODUCTION: Neonatal testicular germ cells/gonocytes, transform into stem cells for spermatogenesis during 'minipuberty', driving change in timing of surgery. This study examined gonocyte transformation in cryptorchid testes in children ≤18 months of age with unilateral, bilateral undescended testes (UDT), complete or partial androgen insensitivity syndrome (CAIS, PAIS) [3,4].

MATERIAL AND METHODS: Testicular biopsies were taken from patients with unilateral or bilateral UDT, PAIS or CAIS, aged 10 days-18 months. These testicular sections underwent immunohistochemistry with antibodies (Oct4, Ki67, C-Kit, Sox9) followed by confocal imaging, cell counting and statistical analysis.

RESULTS: Both Sertoli cells/tubule and germ cells (GC)/tubule decreased with age, and % empty tubules (no GC) increased with age but with no significant differences between patient groups. Oct4+ germ cells/tubule decreased with age. There are some GCs and Sertoli cells proliferating during the first year and most proliferating Oct4+ germ cells (Oct4+/Ki67+) were located off tubular basement membrane.

CONCLUSION: Our study showed that Oct4 expression gradually decreased after minipuberty and transformation into spermatogonia. Germ cells and Sertoli cells undergo mitosis during the first 12 months although not abundantly. We propose that Oct4+ gonocyte transformation into spermatogonia via proliferation and migration to the basement membrane may be delayed in UDT.

OriginalsprogEngelsk
TidsskriftJournal of Pediatric Surgery
Vol/bind54
Udgave nummer8
Sider (fra-til)1654-1659
Antal sider6
ISSN0022-3468
DOI
StatusUdgivet - 1 aug. 2019

ID: 56518908