Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Harvard

Alatraktchi, FA, Noori, JS, Tanev, GP, Mortensen, J, Dimaki, M, Johansen, HK, Madsen, J, Molin, S & Svendsen, WE 2018, 'Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa' PLoS One, bind 13, nr. 3, s. e0194157. https://doi.org/10.1371/journal.pone.0194157

APA

Alatraktchi, F. A., Noori, J. S., Tanev, G. P., Mortensen, J., Dimaki, M., Johansen, H. K., ... Svendsen, W. E. (2018). Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa. PLoS One, 13(3), e0194157. https://doi.org/10.1371/journal.pone.0194157

CBE

Alatraktchi FA, Noori JS, Tanev GP, Mortensen J, Dimaki M, Johansen HK, Madsen J, Molin S, Svendsen WE. 2018. Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa. PLoS One. 13(3):e0194157. https://doi.org/10.1371/journal.pone.0194157

MLA

Vancouver

Author

Alatraktchi, Fatima AlZahra'a ; Noori, Jafar Safaa ; Tanev, Georgi Plamenov ; Mortensen, John ; Dimaki, Maria ; Johansen, Helle Krogh ; Madsen, Jan ; Molin, Søren ; Svendsen, Winnie E. / Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa. I: PLoS One. 2018 ; Bind 13, Nr. 3. s. e0194157.

Bibtex

@article{2125d0a6a5f74f0ab0860a76a8b8c14b,
title = "Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa",
abstract = "Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance. The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.",
keywords = "Biosensing Techniques, Humans, Paper, Pseudomonas Infections/diagnosis, Pseudomonas aeruginosa/metabolism, Pyocyanine/analysis, Sensitivity and Specificity, Virulence Factors/analysis",
author = "Alatraktchi, {Fatima AlZahra'a} and Noori, {Jafar Safaa} and Tanev, {Georgi Plamenov} and John Mortensen and Maria Dimaki and Johansen, {Helle Krogh} and Jan Madsen and S{\o}ren Molin and Svendsen, {Winnie E}",
year = "2018",
doi = "10.1371/journal.pone.0194157",
language = "English",
volume = "13",
pages = "e0194157",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosa

AU - Alatraktchi, Fatima AlZahra'a

AU - Noori, Jafar Safaa

AU - Tanev, Georgi Plamenov

AU - Mortensen, John

AU - Dimaki, Maria

AU - Johansen, Helle Krogh

AU - Madsen, Jan

AU - Molin, Søren

AU - Svendsen, Winnie E

PY - 2018

Y1 - 2018

N2 - Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance. The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.

AB - Pyocyanin is a toxin produced by Pseudomonas aeruginosa. Here we describe a novel paper-based electrochemical sensor for pyocyanin detection, manufactured with a simple and inexpensive approach based on electrode printing on paper. The resulting sensors constitute an effective electrochemical method to quantify pyocyanin in bacterial cultures without the conventional time consuming pretreatment of the samples. The electrochemical properties of the paper-based sensors were evaluated by ferri/ferrocyanide as a redox mediator, and showed reliable sensing performance. The paper-based sensors readily allow for the determination of pyocyanin in bacterial cultures with high reproducibility, achieving a limit of detection of 95 nM and a sensitivity of 4.30 μA/μM in standard culture media. Compared to the similar commercial ceramic based sensors, it is a 2.3-fold enhanced performance. The simple in-house fabrication of sensors for pyocyanin quantification allows researchers to understand in vitro adaptation of P. aeruginosa infections via rapid screenings of bacterial cultures that otherwise are expensive and time-consuming.

KW - Biosensing Techniques

KW - Humans

KW - Paper

KW - Pseudomonas Infections/diagnosis

KW - Pseudomonas aeruginosa/metabolism

KW - Pyocyanine/analysis

KW - Sensitivity and Specificity

KW - Virulence Factors/analysis

U2 - 10.1371/journal.pone.0194157

DO - 10.1371/journal.pone.0194157

M3 - Journal article

VL - 13

SP - e0194157

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 3

ER -

ID: 56201764