Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

P2X7Rs are involved in cell death, growth and cellular signaling in primary human osteoblasts

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Possible link between FSH and RANKL release from adipocytes in men with impaired gonadal function including Klinefelter syndrome

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Bone mass development is sensitive to insulin resistance in adolescent boys

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Effects of metformin, rosiglitazone and insulin on bone metabolism in patients with type 2 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Determinants of vitamin D status in a general population of Danish adults

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. ZP2307, a novel, cyclic PTH(1-17) analog that augments bone mass in ovariectomized rats

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Multiple Fractures and Impaired Bone Fracture Healing in a Patient with Pycnodysostosis and Hypophosphatasia

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Assessment of acute bone loading in humans using [18F]NaF PET/MRI

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Bone turnover markers in children and adolescents with type 1 diabetes-A systematic review

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

Vis graf over relationer

The ionotropic ATP-gated P2X7 receptor (P2X7R) is involved in the regulation of many physiological functions including bone metabolism. Several studies on osteoblasts from rodents and human osteoblast-like cell lines have addressed the expression and function of P2X7R on these bone-forming cells however; its role in human primary osteoblasts has not yet been reported. The aim of this study was to assess the expression of the P2X7R in bone marrow-derived stromal cells and in primary human trabecular osteoblasts and to determine the function in bone formation and cell signaling. We report that osteoblasts derived from human trabecular explants express a functional P2X7R capable of agonist-induced increase in intracellular calcium concentration and a positive permeability to fluorescent dyes. These osteoblasts are fully differentiated cells with alkaline phosphatase activity and the ability to form mineralized nodules. We show that the transcriptional regulation of osteoblastic markers can be modulated by P2X7R activity or blockade thereby influencing the differentiation, proliferation and bone matrix formation by these primary human osteoblasts. Finally, we demonstrate that the P2X7R is involved in propagation of mechanically-induced intercellular signaling in addition to the known mechanisms involving calcium signaling via P2Y2 receptors and gap junction.

OriginalsprogEngelsk
TidsskriftBone
Vol/bind95
Sider (fra-til)91-101
ISSN8756-3282
DOI
StatusUdgivet - feb. 2017

ID: 49260552