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Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs

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Harvard

Ludvigsen, TP, Wiinberg, N, Jensen, CJ, Callesen, AT, Andersen, RW, Jørgensen, ASH, Christoffersen, BØ, Pedersen, HD, Moesgaard, SG & Olsen, LH 2014, 'Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs' Comparative Medicine, bind 64, nr. 6, s. 471-7.

APA

Ludvigsen, T. P., Wiinberg, N., Jensen, C. J., Callesen, A. T., Andersen, R. W., Jørgensen, A. S. H., ... Olsen, L. H. (2014). Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs. Comparative Medicine, 64(6), 471-7.

CBE

Ludvigsen TP, Wiinberg N, Jensen CJ, Callesen AT, Andersen RW, Jørgensen ASH, Christoffersen BØ, Pedersen HD, Moesgaard SG, Olsen LH. 2014. Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs. Comparative Medicine. 64(6):471-7.

MLA

Vancouver

Author

Ludvigsen, Trine P ; Wiinberg, Niels ; Jensen, Christina J ; Callesen, Annemette T ; Andersen, Regitze W ; Jørgensen, Anne Sofie H ; Christoffersen, Berit Ø ; Pedersen, Henrik D ; Moesgaard, Sophia G ; Olsen, Lisbeth H. / Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs. I: Comparative Medicine. 2014 ; Bind 64, Nr. 6. s. 471-7.

Bibtex

@article{85376612feda44ab92b1026e12b15868,
title = "Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized G{\"o}ttingen minipigs",
abstract = "Few methods for noninvasive assessment of arterial stiffness and endothelial dysfunction in porcine models are available. The aim of this study was to evaluate methods for assessment of arterial stiffness and endothelial dysfunction in anesthetized G{\"o}ttingen minipigs. Pulse-wave velocity (PWV) was assessed in male G{\"o}ttingen minipigs (n = 8; age approximately 60 wk) by using applanation tonometry of the carotid and femoral arteries. In addition, flow-mediated vasodilation (FMD) was assessed by using vascular ultrasonography of the brachial artery to evaluate endothelial dysfunction. To evaluate the reproducibility of the methods, minipigs were anesthetized by intravenous infusion of ketamine and midazolam and examined every other day for a total of 3 trials. Neither examination day nor systolic, diastolic, or mean arterial blood pressure statistically influenced PWV or FMD. The median interexamination coefficient of variation was 17{\%} for PWV and 59{\%} for FMD. Measured values of PWV corresponded largely to those in clinically healthy humans, but FMD values were lower than expected for lean, young animals. Although the ketamine-midazolam anesthesia we used has been associated with minor hemodynamic effects in vivo, in vitro studies suggest that both drugs are vasodilatory. Therefore anesthesia might have influenced the endothelial response, contributing to the modest FMD response and the concurrent high coefficients of variation that we noted. We conclude that PWV—but not FMD—showed acceptable interexamination variation for its potential application in porcine models.",
keywords = "Anesthesia, Animals, Blood Pressure, Endothelium/physiopathology, Ketamine, Male, Midazolam, Pulse Wave Analysis/methods, Swine, Swine, Miniature/physiology, Vascular Stiffness/physiology, Vasodilation/physiology",
author = "Ludvigsen, {Trine P} and Niels Wiinberg and Jensen, {Christina J} and Callesen, {Annemette T} and Andersen, {Regitze W} and J{\o}rgensen, {Anne Sofie H} and Christoffersen, {Berit {\O}} and Pedersen, {Henrik D} and Moesgaard, {Sophia G} and Olsen, {Lisbeth H}",
year = "2014",
month = "12",
language = "English",
volume = "64",
pages = "471--7",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "6",

}

RIS

TY - JOUR

T1 - Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs

AU - Ludvigsen, Trine P

AU - Wiinberg, Niels

AU - Jensen, Christina J

AU - Callesen, Annemette T

AU - Andersen, Regitze W

AU - Jørgensen, Anne Sofie H

AU - Christoffersen, Berit Ø

AU - Pedersen, Henrik D

AU - Moesgaard, Sophia G

AU - Olsen, Lisbeth H

PY - 2014/12

Y1 - 2014/12

N2 - Few methods for noninvasive assessment of arterial stiffness and endothelial dysfunction in porcine models are available. The aim of this study was to evaluate methods for assessment of arterial stiffness and endothelial dysfunction in anesthetized Göttingen minipigs. Pulse-wave velocity (PWV) was assessed in male Göttingen minipigs (n = 8; age approximately 60 wk) by using applanation tonometry of the carotid and femoral arteries. In addition, flow-mediated vasodilation (FMD) was assessed by using vascular ultrasonography of the brachial artery to evaluate endothelial dysfunction. To evaluate the reproducibility of the methods, minipigs were anesthetized by intravenous infusion of ketamine and midazolam and examined every other day for a total of 3 trials. Neither examination day nor systolic, diastolic, or mean arterial blood pressure statistically influenced PWV or FMD. The median interexamination coefficient of variation was 17% for PWV and 59% for FMD. Measured values of PWV corresponded largely to those in clinically healthy humans, but FMD values were lower than expected for lean, young animals. Although the ketamine-midazolam anesthesia we used has been associated with minor hemodynamic effects in vivo, in vitro studies suggest that both drugs are vasodilatory. Therefore anesthesia might have influenced the endothelial response, contributing to the modest FMD response and the concurrent high coefficients of variation that we noted. We conclude that PWV—but not FMD—showed acceptable interexamination variation for its potential application in porcine models.

AB - Few methods for noninvasive assessment of arterial stiffness and endothelial dysfunction in porcine models are available. The aim of this study was to evaluate methods for assessment of arterial stiffness and endothelial dysfunction in anesthetized Göttingen minipigs. Pulse-wave velocity (PWV) was assessed in male Göttingen minipigs (n = 8; age approximately 60 wk) by using applanation tonometry of the carotid and femoral arteries. In addition, flow-mediated vasodilation (FMD) was assessed by using vascular ultrasonography of the brachial artery to evaluate endothelial dysfunction. To evaluate the reproducibility of the methods, minipigs were anesthetized by intravenous infusion of ketamine and midazolam and examined every other day for a total of 3 trials. Neither examination day nor systolic, diastolic, or mean arterial blood pressure statistically influenced PWV or FMD. The median interexamination coefficient of variation was 17% for PWV and 59% for FMD. Measured values of PWV corresponded largely to those in clinically healthy humans, but FMD values were lower than expected for lean, young animals. Although the ketamine-midazolam anesthesia we used has been associated with minor hemodynamic effects in vivo, in vitro studies suggest that both drugs are vasodilatory. Therefore anesthesia might have influenced the endothelial response, contributing to the modest FMD response and the concurrent high coefficients of variation that we noted. We conclude that PWV—but not FMD—showed acceptable interexamination variation for its potential application in porcine models.

KW - Anesthesia

KW - Animals

KW - Blood Pressure

KW - Endothelium/physiopathology

KW - Ketamine

KW - Male

KW - Midazolam

KW - Pulse Wave Analysis/methods

KW - Swine

KW - Swine, Miniature/physiology

KW - Vascular Stiffness/physiology

KW - Vasodilation/physiology

M3 - Journal article

VL - 64

SP - 471

EP - 477

JO - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 6

ER -

ID: 59054135