Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Impaired skeletal muscle hypertrophy signaling and amino acid deprivation response in Apoe knockout mice with an unhealthy lipoprotein distribution

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Infants with congenital heart defects have reduced brain volumes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Instrumental variable analysis using offspring BMI in childhood as an indicator of parental BMI in relation to mortality

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. De novo electrocardiographic abnormalities in persons living with HIV

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Using machine learning for predicting intensive care unit resource use during the COVID-19 pandemic in Denmark

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Effect of liraglutide on expression of inflammatory genes in type 2 diabetes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Jakob Agergaard
  • Mie Cecilie Faber Zillmer
  • Josué L Castro-Mejía
  • Kenneth Mertz
  • Witold Kot
  • Grith Højfeldt
  • Gerrit van Hall
  • Dennis S Nielsen
  • Peter Schjerling
  • Lars Holm
Vis graf over relationer

This study explores if unhealthy lipoprotein distribution (LPD) impairs the anabolic and amino acid sensing responses to whey-protein feeding. Thus, if impairment of such anabolic response to protein consumption is seen by the LPD this may negatively affect the skeletal muscle mass. Muscle protein synthesis (MPS) was measured by puromycin labeling in Apolipoprotein E knockout (Apoe KO), characterized by an unhealthy LPD, and wild type mice post-absorptive at 10 and 20 weeks, and post-prandial after whey-protein feeding at 20 weeks. Hypertrophy signaling and amino acid sensing mechanisms were studied and gut microbiome diversity explored. Surprisingly, whey-protein feeding did not affect MPS. p-mTOR and p-4E-BP1 was increased 2 h after whey-protein feeding in both genotypes, but with general lower levels in Apoe KO compared to wild type. At 20 weeks of age, Apoe KO had a greater mRNA-expression for SNAT2, CD98, ATF4 and GCN2 compared to wild type. These responses were not associated with gut microbiota compositional differences. Regardless of LPD status, MPS was similar in Apoe KO and wild type. Surprisingly, whey-protein did not stimulate MPS. However, Apoe KO had lower levels of hypertrophy signaling, was amino acid deprived, and had impaired amino acid sensing mechanisms.

OriginalsprogEngelsk
Artikelnummer16423
TidsskriftScientific Reports
Vol/bind11
Udgave nummer1
ISSN2045-2322
DOI
StatusUdgivet - 12 aug. 2021

ID: 67244665