Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
AIM: To define the genomic distribution and function of DNA methylation changes during human adipogenesis.
METHODS: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes.
RESULTS: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling.
CONCLUSION: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Epigenomics |
| Vol/bind | 8 |
| Udgave nummer | 12 |
| Sider (fra-til) | 1601-1617 |
| Antal sider | 17 |
| ISSN | 1750-1911 |
| DOI | |
| Status | Udgivet - dec. 2016 |
ID: 49913935