Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Heart ischaemia-reperfusion induces local up-regulation of vasoconstrictor endothelin ETB receptors in rat coronary arteries downstream of occlusion

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. PACAP and its receptors in migraine pathophysiology

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Angiotensin II type 1 receptor signalling regulates microRNA differentially in cardiac fibroblasts and myocytes

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. The calcium-sensing receptor and calcimimetics in blood pressure modulation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. beta-Arrestin 1 and 2 stabilize the angiotensin II type I receptor in distinct high-affinity conformations

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Effects of ionotropic glutamate receptor antagonists on rat dural artery diameter in an intravital microscopy model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Does inflammation have a role in migraine?

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  2. The changing faces of migraine

    Publikation: Bidrag til tidsskriftLederForskningpeer review

  3. CGRP receptor antagonist MK-8825 attenuates cortical spreading depression induced pain behavior

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

BACKGROUND AND PURPOSE: Endothelins act via two receptor subtypes, ETA and ETB . Under physiological conditions in coronary arteries, ETA receptors expressed in smooth muscle cells mediate vasoconstriction whereas ETB receptors mainly found in endothelial cells mediate vasorelaxation. However, under pathophysiological conditions, ETB receptors may also be expressed in vascular smooth muscle cells mediating vasoconstriction. Here, we have investigated whether vasoconstrictor ETB receptors are up-regulated in coronary arteries after experimental myocardial ischaemia in rats.

EXPERIMENTAL APPROACH: Male Sprague-Dawley rats were subjected to either heart ischaemia-reperfusion (15 min ischaemia and 22 h reperfusion), permanent ischaemia (22 h) by ligation of the left anterior descending coronary artery, or sham operation. Using wire myography, the endothelin receptor subtypes mediating vasoconstriction were examined in isolated segments of the left anterior descending and the non-ligated septal coronary arteries. Endothelin receptor-mediated vasoconstriction was examined with cumulative administration of sarafotoxin 6c (ETB receptor agonist) and endothelin-1 (with or without ETA or ETB receptor blockade). The distribution of ETB receptors was localized with immunohistochemistry and quantified by Western blot.

KEY RESULTS: Endothelin ETB receptor-mediated vasoconstriction and receptor protein levels were significantly augmented in coronary arteries situated downstream of the occlusion after ischaemia-reperfusion compared with non-ischaemic arteries. In contrast, the ETA receptor-mediated vasoconstriction was unaltered in all groups.

CONCLUSIONS AND IMPLICATIONS: Ischaemia-reperfusion induced local up-regulation of ETB receptors in the smooth muscle cells of coronary arteries in the post-ischaemic area. In contrast, in non-ischaemic areas, ETB receptor function was unaltered.

Bidragets oversatte titelHjerte iskæmi-reperfusion inducerer lokal opregulering af vasokonstriktoriske endothelin ETB receptorer i rotte koronararterier nedstrøm for okklusion
OriginalsprogEngelsk
TidsskriftBritish Journal of Pharmacology
Vol/bind171
Udgave nummer11
Sider (fra-til)2726-38
Antal sider13
ISSN0007-1188
DOI
StatusUdgivet - jun. 2014

ID: 44586987