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Gonocyte transformation in congenital undescended testes: what is the role of inhibin-B in cell death?

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. The impact of early and successful orchidopexy on hormonal follow-up for 208 boys with bilateral non-syndromic cryptorchidism

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  2. Germ cells positive for PLAP and c-Kit in 11-16 year old normal boys with ongoing spermatogenesis

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  3. The relation of germ cells per tubule in testes, serum inhibin B and FSH in cryptorchid boys

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  4. Is infertility after surgery for cryptorchidism congenital or acquired?

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  1. Intestinal perfusion assessed by quantitative fluorescence angiography in piglets with necrotizing enterocolitis

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  2. Characterization and Survival of Human Infant Testicular Cells After Direct Xenotransplantation

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  3. Fertility preservation in boys facing gonadotoxic cancer therapy

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  4. Serial Inhibin B Measurements in Boys with Congenital Monorchism Indicate Compensatory Testicular Hypertrophy in Early Infancy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Vanessa Wilson
  • Jorgen Thorup
  • Erick Clasen-Linde
  • Dina Cortes
  • John M Hutson
  • Ruili Li
Vis graf over relationer

PURPOSE: Undescended testes (UDT) are subjected to heat stress, which can disturb gonocyte transformation as well as apoptosis. This study aims to describe the apoptosis pathway occurring during minipuberty of children with unilateral (UDT), and to investigate the role of inhibin-B.

METHODS: Testicular biopsies at unilateral orchidopexy of 10 boys (6-9 months old) with normal inhibin-B (n = 5) or low inhibin-B (n = 5) were selected for immunohistochemistry and TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labelling) assay. Testicular tubules were labelled with antibodies against Anti-Müllerian hormone (AMH, Sertoli cell marker), mouse Vasa Homolog (MVH) and placental alkaline phosphatase (PLAP) (both germ cell markers), cleaved caspase3 (apoptotic marker), and followed by confocal imaging and cell counting with Fiji/ImageJ. Data were analyzed with GraphPad Prism.

RESULTS: In males with low and normal inhibin-B, there was no statistical difference (p > 0.05) in the percentage of testicular tubules containing TUNEL + cells, number of cleaved caspase3 ± germ cells/tubule, total number of germ cells/tubule, and the percentage of fibrotic tubules or number of Sertoli cells/tubule.

CONCLUSIONS: These results suggest that inhibin-B does not regulate cell death of gonocytes and further studies are required to uncover any role of inhibin-B in gonocyte transformation.

OriginalsprogEngelsk
TidsskriftPediatric Surgery International
Vol/bind35
Udgave nummer11
Sider (fra-til)1309-1316
ISSN0179-0358
DOI
StatusUdgivet - nov. 2019

ID: 57847717