Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Gastrointestinal microbiota and local inflammation during oxazolone-induced dermatitis in BALB/cA mice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Effect of Early-life Gut Mucosal Compromise on Disease Progression in NOD Mice

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Antibiotic treatment during early childhood and risk of type 1 diabetes in children: A national birth cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Investigating the aetiology of adverse events following HPV vaccination with systems vaccinology

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. Possible Prevention of Diabetes with a Gluten-Free Diet

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  4. Association between maternal gluten intake and type 1 diabetes in offspring: national prospective cohort study in Denmark

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Gluten-free diet during pregnancy alleviates signs of diabetes and celiac disease in NOD mouse offspring

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Randi Lundberg
  • Susanne Clausen
  • Wanyong Pang
  • Dennis Nielsen
  • Kristian Möller
  • Knud E Josefsen
  • Axel K Hansen
Vis graf over relationer
At present, laboratory animals are not standardized with regard to the gastrointestinal microbiota (GM), but differences in this feature may alter various parameters in animal models. We hypothesized that variation in the GM correlated with variation in clinical parameters of a murine oxazolone-induced skin inflammation model of atopic dermatitis. BALB/cA mice were sensitized with oxazolone over a 28-d period and variation in gastrointestinal microbiota in fecal and cecal samples was assessed by PCR-denaturing gradient gel electrophoresis. Clinical parameters included transepidermal water loss, ear thickness, inflammatory factors in ear tissue and plasma, and histopathologic evaluation. The fecal microbiota before induction of skin inflammation strongly correlated with the levels of some proinflammatory cytokines (IFNγ, IL1β, IL12, and TNFα), the antiinflammatory cytokines IL4 and IL10, and the chemokine KC/GRO that were measured in ear samples at study termination. Cecal microbiota at termination correlated with ear thickness and transepidermal water loss. There was no correlation between cytokine responses and ear thickness or transepidermal water loss. In addition, GM changed during the study period in the oxazolone-treated mice, whereas this was not the case for the control mice. The current study shows that the GM of mice influences the development of oxazolone-induced skin inflammation and that the model itself likely induces a pathophysiologic response that alters the composition of the GM.
OriginalsprogEngelsk
TidsskriftComparative Medicine (Memphis)
Vol/bind62
Udgave nummer5
Sider (fra-til)371-80
Antal sider10
ISSN1532-0820
StatusUdgivet - 2012

ID: 36847908