Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

GABAA Receptor-Mediated Bidirectional Control of Synaptic Activity, Intracellular Ca2+, Cerebral Blood Flow, and Oxygen Consumption in Mouse Somatosensory Cortex In Vivo

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Human Cerebral Perfusion, Oxygen Consumption, and Lactate Production in Response to Hypoxic Exposure

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Interneuron Deficit Associates Attenuated Network Synchronization to Mismatch of Energy Supply and Demand in Aging Mouse Brains

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Neocortical Neuronal Loss in Patients with Multiple System Atrophy: A Stereological Study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Reduced GABAergic inhibition explains cortical hyperexcitability in the wobbler mouse model of ALS

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Monocular visual deprivation suppresses excitability in adult human visual cortex

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Automatic continuous EEG signal analysis for diagnosis of delirium in patients with sepsis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Post-capillary venules are the key locus for transcytosis-mediated brain delivery of therapeutic nanoparticles

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Precapillary sphincters and pericytes at first-order capillaries as key regulators for brain capillary perfusion

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Brain capillary pericytes and neurovascular coupling

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  5. ATP induces contraction of cultured brain capillary pericytes via activation of P2Y-type purinergic receptors

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Sanne Barsballe Jessen
  • Alexey Brazhe
  • Barbara Lykke Lind
  • Claus Mathiesen
  • Kirsten Thomsen
  • Kimmo Jensen
  • Martin Lauritzen
Vis graf over relationer

Neural activity regulates local increases in cerebral blood flow (ΔCBF) and the cortical metabolic rate of oxygen (ΔCMRO2) that constitutes the basis of BOLD functional neuroimaging signals. Glutamate signaling plays a key role in brain vascular and metabolic control; however, the modulatory effect of GABA is incompletely understood. Here we performed in vivo studies in mice to investigate how THIP (which tonically activates extrasynaptic GABAARs) and Zolpidem (a positive allosteric modulator of synaptic GABAARs) impact stimulation-induced ΔCBF, ΔCMRO2, local field potentials (LFPs), and fluorescent cytosolic Ca(2+) transients in neurons and astrocytes. Low concentrations of THIP increased ΔCBF and ΔCMRO2 at low stimulation frequencies. These responses were coupled to increased synaptic activity as indicated by LFP responses, and to Ca(2+) activities in neurons and astrocytes. Intermediate and high concentrations of THIP suppressed ΔCBF and ΔCMRO2 at high stimulation frequencies. Zolpidem had similar but less-pronounced effects, with similar dependence on drug concentration and stimulation frequency. Our present findings suggest that slight increases in both synaptic and extrasynaptic GABAAR activity might selectively gate and amplify transient low-frequency somatosensory inputs, filter out high-frequency inputs, and enhance vascular and metabolic responses that are likely to be reflected in BOLD functional neuroimaging signals.

OriginalsprogEngelsk
TidsskriftCerebral Cortex
Vol/bind25
Udgave nummer9
Sider (fra-til)2594-609
Antal sider16
ISSN1047-3211
DOI
StatusUdgivet - sep. 2015

ID: 45363745