Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Flow cytometry analysis of T-cell subsets in cerebrospinal fluid of narcolepsy type 1 patients with long-lasting disease

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Assessment of diurnal melatonin, cortisol, activity, and sleep-wake cycle in patients with and without diabetic retinopathy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Melatonin and cortisol in individuals with spinal cord injury

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Restless legs syndrome is associated with major comorbidities in a population of Danish blood donors

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Mortality and use of psychotropic medication in sleep apnoea patients: a population-wide register-based study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. A comparative study of methods for automatic detection of rapid eye movement abnormal muscular activity in narcolepsy

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Type 1 narcolepsy is not present in 29 HPV-vaccinated individuals with subjective sleep complaints

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. DNMT1 regulates expression of MHC class i in post-mitotic neurons

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. N-terminal tagging of human P2X7 receptor disturbs calcium influx and dye uptake

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Increased interferon-mediated immunity following in vitro and in vivo Modafinil treatment on peripheral immune cells

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  • Monica Moresco
  • Mariangela Lecciso
  • Darina Ocadlikova
  • Marco Filardi
  • Silvia Melzi
  • Birgitte Rahbek Kornum
  • Elena Antelmi
  • Fabio Pizza
  • Emmanuel Mignot
  • Antonio Curti
  • Giuseppe Plazzi
Vis graf over relationer

Background: Type 1 narcolepsy (NT1) is a central hypersomnia linked to the destruction of hypocretin-producing neurons. A great body of genetic and epidemiological data points to likely autoimmune disease aetiology. Recent reports have characterized peripheral blood T-cell subsets in NT1, whereas data regarding the cerebrospinal fluid (CSF) immune cell composition are lacking. The current study aimed to characterize the T-cell and natural killer (NK) cell subsets in NT1 patients with long disease course. Methods: Immune cell subsets from CSF and peripheral blood mononuclear cell (PBMC) samples were analysed by flow cytometry in two age-balanced and sex-balanced groups of 14 NT1 patients versus 14 healthy controls. The frequency of CSF cell groups was compared with PBMCs. Non-parametric tests were used for statistical analyses. Results: The NT1 patients did not show significant differences of CSF immune cell subsets compared to controls, despite a trend towards higher CD4+ terminally differentiated effector memory T cells. T cells preferentially displayed a memory phenotype in the CSF compared to PBMCs. Furthermore, a reduced frequency of CD4+ terminally differentiated effector memory T cells and an increased frequency of NK CD56bright cells was observed in PBMCs from patients compared to controls. Finally, the ratio between CSF and peripheral CD4+ terminally differentiated effector memory T cells was two-fold increased in NT1 patients versus controls. Conclusions: Significant differences in PBMCs and in CSF/PBMC ratios of immune cell profile were found in NT1 patients compared to healthy controls. These differences might have arisen from the different HLA status, or be primary or secondary to hypocretin deficiency. Further functional studies in patients close to disease onset are required to understand NT1 pathophysiology.

OriginalsprogEngelsk
TidsskriftSleep Medicine
Vol/bind44
Sider (fra-til)53-60
Antal sider8
ISSN1389-9457
DOI
StatusUdgivet - 1 apr. 2018

ID: 55697233