Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Expression of the MexXY Aminoglycoside Efflux Pump and Presence of an Aminoglycoside-Modifying Enzyme in Clinical Pseudomonas aeruginosa Isolates Are Highly Correlated

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Evolution of Pseudomonas aeruginosa toward higher fitness under standard laboratory conditions

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Genetic determinants of Pseudomonas aeruginosa fitness during biofilm growth

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Targeting bioenergetics is key to counteracting the drug-tolerant state of biofilm-grown bacteria

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Mining zebrafish microbiota reveals key community-level resistance against fish pathogen infection

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

The impact of MexXY efflux pump expression on aminoglycoside resistance in clinical Pseudomonas aeruginosa isolates has been debated. In this study, we found that, in general, elevated mexXY gene expression levels in clinical P. aeruginosa isolates confer to slight increases in aminoglycoside MIC levels; however, those levels rarely lead to clinically relevant resistance phenotypes. The main driver of resistance in the clinical isolates studied here was the acquisition of aminoglycoside-modifying enzymes (AMEs). Nevertheless, acquisition of an AME was strongly associated with mexY overexpression. In line with this observation, we demonstrate that the introduction of a gentamicin acetyltransferase confers to full gentamicin resistance levels in a P. aeruginosa type strain only if the MexXY efflux pump was active. We discuss that increased mexXY activity in clinical AME-harboring P. aeruginosa isolates might affect ion fluxes at the bacterial cell membrane and thus might play a role in the establishment of enhanced fitness that extends beyond aminoglycoside resistance.

OriginalsprogEngelsk
Artikelnummere01166-20
TidsskriftAntimicrobial Agents and Chemotherapy
Vol/bind65
Udgave nummer1
ISSN0066-4804
DOI
StatusUdgivet - jan. 2021

Bibliografisk note

Copyright © 2020 Seupt et al.

ID: 62339636