Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Environmental heterogeneity drives within-host diversification and evolution of Pseudomonas aeruginosa

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

DOI

  1. Convergent Metabolic Specialization through Distinct Evolutionary Paths in Pseudomonas aeruginosa

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. The Pseudomonas aeruginosa PSL Polysaccharide Is a Social but Noncheatable Trait in Biofilms

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Phage Inhibit Pathogen Dissemination by Targeting Bacterial Migrants in a Chronic Infection Model

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Role of Multicellular Aggregates in Biofilm Formation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. Plasmodium falciparum-Infected Erythrocyte Knob Density Is Linked to the PfEMP1 Variant Expressed

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Adjunctive dabigatran therapy improves outcome of experimental left-sided Staphylococcus aureus endocarditis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Antibody response against Mycobacterium avium complex in cystic fibrosis patients measured by a novel IgG ELISA test

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Privatisation rescues function following loss of cooperation

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. The importance of early diagnosis of Mycobacterium abscessus complex in patients with cystic fibrosis

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  5. Treatment of candidemia in a nationwide setting: increased survival with primary echinocandin treatment

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

UNLABELLED: Microbial population polymorphisms are commonly observed in natural environments, including long-term infected hosts. However, the underlying processes promoting and stabilizing diversity are difficult to unravel and are not well understood. Here, we use chronic infection of cystic fibrosis airways by the opportunistic pathogen Pseudomonas aeruginosa as a system for investigating bacterial diversification processes during the course of infection. We analyze clonal bacterial isolates sampled during a 32-year period and map temporal and spatial variations in population diversity to different infection sites within the infected host. We show that the ancestral infecting strain diverged into distinct sublineages, each with their own functional and genomic signatures and rates of adaptation, immediately after initial colonization. The sublineages coexisted in the host for decades, suggesting rapid evolution of stable population polymorphisms. Critically, the observed generation and maintenance of population diversity was the result of partitioning of the sublineages into physically separated niches in the CF airway. The results reveal a complex within-host population structure not previously realized and provide evidence that the heterogeneity of the highly structured and complex host environment promotes the evolution and long-term stability of pathogen population diversity during infection.

IMPORTANCE: Within-host pathogen evolution and diversification during the course of chronic infections is of importance in relation to therapeutic intervention strategies, yet our understanding of these processes is limited. Here, we investigate intraclonal population diversity in P. aeruginosa during chronic airway infections in cystic fibrosis patients. We show the evolution of a diverse population structure immediately after initial colonization, with divergence into multiple distinct sublineages that coexisted for decades and occupied distinct niches. Our results suggest that the spatial heterogeneity in CF airways plays a major role in relation to the generation and maintenance of population diversity and emphasize that a single isolate in sputum may not represent the entire pathogen population in the infected individual. A more complete understanding of the evolution of distinct clonal variants and their distribution in different niches could have positive implications for efficient therapy.

OriginalsprogEngelsk
TidsskriftmBio
Vol/bind5
Udgave nummer5
Sider (fra-til)e01592-14
ISSN2161-2129
DOI
StatusUdgivet - 2014

ID: 44828470