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Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling

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  2. Transcriptome-Wide Association Study Identifies New Candidate Susceptibility Genes for Glioma

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  3. A large-cohort, longitudinal study determines pre-cancer disease routes across different cancer types

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  4. Genetic data from nearly 63,000 women of European descent predicts DNA methylation biomarkers and epithelial ovarian cancer risk

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  1. Novel functions of the luteinizing hormone/chorionic gonadotropin receptor in prostate cancer cells and patients

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  2. 'Snail factors in testicular germ cell tumours and their regulation by the BMP4 signalling pathway'

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  3. Activin A determines steroid levels and composition in the fetal testis

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  4. Distinguishing between hidden testes and anorchia: The role of endocrine evaluation in infancy and childhood

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Vis graf over relationer
Carcinoma in situ (CIS) is the common precursor of histologically heterogeneous testicular germ cell tumors (TGCTs), which in recent decades have markedly increased and now are the most common malignancy of young men. Using genome-wide gene expression profiling, we identified >200 genes highly expressed in testicular CIS, including many never reported in testicular neoplasms. Expression was further verified by semiquantitative reverse transcription-PCR and in situ hybridization. Among the highest expressed genes were NANOG and POU5F1, and reverse transcription-PCR revealed possible changes in their stoichiometry on progression into embryonic carcinoma. We compared the CIS expression profile with patterns reported in embryonic stem cells (ESCs), which revealed a substantial overlap that may be as high as 50%. We also demonstrated an over-representation of expressed genes in regions of 17q and 12, reported as unstable in cultured ESCs. The close similarity between CIS and ESCs explains the pluripotency of CIS. Moreover, the findings are consistent with an early prenatal origin of TGCTs and thus suggest that etiologic factors operating in utero are of primary importance for the incidence trends of TGCTs. Finally, some of the highly expressed genes identified in this study are promising candidates for new diagnostic markers for CIS and/or TGCTs.
OriginalsprogEngelsk
TidsskriftCancer Research
Vol/bind64
Udgave nummer14
Sider (fra-til)4736-43
Antal sider8
ISSN0008-5472
DOI
StatusUdgivet - 15 jul. 2004

ID: 41682117