Forskning
Udskriv Udskriv
Switch language
Rigshospitalet - en del af Københavns Universitetshospital
Udgivet

Diagnosis of biofilm infections in cystic fibrosis patients

Publikation: Bidrag til tidsskriftReviewForskningpeer review

DOI

  1. Primary antibiotic resistance of Helicobacter pylori strains among adults and children in a tertiary referral centre in Lithuania

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. A short history of microbial biofilms and biofilm infections

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  3. Biofilms and host response - helpful or harmful

    Publikation: Bidrag til tidsskriftReviewForskningpeer review

  1. Hydrochloric acid prolongs the lifetime of central venous catheters in haematologic patients with bacteraemia

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Epidemiology of bloodstream infections in patients with chronic lymphocytic leukemia: a longitudinal nation-wide cohort study

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Adjunctive dabigatran therapy improves outcome of experimental left-sided Staphylococcus aureus endocarditis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Antibody response against Mycobacterium avium complex in cystic fibrosis patients measured by a novel IgG ELISA test

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  5. In-vivo gentamicin susceptibility test for prevention of bacterial biofilms in bone tissue and on implants

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer

Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix. Specific PNA-FISH probes can be used to identify P. aeruginosa and other pathogens in situ in the biofilms. Growth of mucoid colonies from the locations mentioned above is also diagnostic for biofilm infection. Rise of specific anti-P. aeruginosa antibodies is likewise diagnostic, IgG in serum in case of lung infection, sIgA in saliva or nasal secretions in case of paranasal sinus infection. Similar approaches have been developed to diagnose chronic biofilm infections in cystic fibrosis caused by other pathogens e.g., Stenotrophomonas, Burkholderia multivorans, Achromobacter xylosoxidans and Mycobacterium abscessus complex.

OriginalsprogEngelsk
TidsskriftAPMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Vol/bind125
Udgave nummer4
Sider (fra-til)339-343
Antal sider5
ISSN0365-5555
DOI
StatusUdgivet - apr. 2017

ID: 51981006