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Approaches for cytogenetic and molecular analyses of small flow-sorted cell populations from childhood leukemia bone marrow samples

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Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.
OriginalsprogEngelsk
TidsskriftJournal of Immunological Methods
Vol/bind369
Udgave nummer1-2
Sider (fra-til)69-73
Antal sider5
ISSN0022-1759
DOI
StatusUdgivet - 2011

ID: 33119548